秋水仙碱处理白杨雌花芽培育三倍体植株的研究  

STUDIES ON OBTAINING TRIPLOIDS BY COLCHICINE TREATING FEMALE FLOWER BUDS OF WHITE POPLAR

作　　者：李云[1] 朱之悌[1] 田砚亭[1] 张志毅[1] 康向阳[1] 

机构地区：[1]北京林业大学生物学院,北京100083

出　　处：《林业科学》2001年第5期68-74,共7页Scientia Silvae Sinicae

基　　金：横向合作科研课题

摘　　要：采用不同浓度的秋水仙碱溶液以及不同方法处理毛新杨、银腺杨、银毛杨雌花芽诱导其雌配子染色体加倍 ,经杂交获得种子后 ,当年播种育苗 ,并在苗期进行表型初选 ,然后对初选的入选群体进行染色体数目镜检 ,其中从表现生长速度快、叶片大而厚、颜色深绿的入选群体中检测出 13株三倍体植株。试验结果表明 :①水培雌花枝 1～ 5d期间是处理雌花芽诱导雌配子染色体加倍的有效时期 ;②三种秋水仙碱溶液处理方法诱导雌配子染色体加倍培育三倍体均有效 ,其中以瓶浸法处理效果较好 ;③秋水仙碱处理浓度以 0 2 5 %和0 5 0 %有效 ,其中以 0 5 0 %较佳。triploids had been obtained by using different concentrations of colchicine solution and different methods for treating female flower buds of \%Populus tomentosa×P.bolleana,P.alba×P.glandulosa\% and \%P.abba×P.tomentosa.\%The results were as follows:①It was a moderate treating time for chromosome doubling of female gamete from female flower buds on the female flower branches which had been cultured in water for 1～5 days.This period of time was a sensitive phase of female gamete that was treated.② 3 methods of treating female flower buds with colchicine solution were effective.The better method was dipping female flower bud in the bottle of colchicine solution.③ The effective colchicine concentrations were 0 25 % and 0.50 %,the better one was 0.50 %.④The selected triploids had rapid growing,gigantic and vigorous properties at seedling period time.

关 键 词：白杨 雌配子 秋水仙碱 三倍体植株 雌花芽 培育 

分 类 号：S792.11[农业科学—林木遗传育种]