铜锌超氧化物歧化酶与丝氨酸钴(Ⅱ)的相互作用  

Study on the Interaction of Copper-Zinc Superoxide Dismutase with Cobalt(Ⅱ)-Serine by Spectral Analysis

作　　者：郑学仿[1,2] 王静云[3] 王园[1] 高大彬[1] 胡皆汉[4] 

机构地区：[1]大连大学生物工程学院 [2]大连理工大学化工学院生物工程系,大连116012 [3]大连理工大学化工学院生物工程系 [4]中国科学院大连化学物理研究所

出　　处：《催化学报》2001年第6期582-584,共3页

基　　金：国家自然科学基金 ( 2 0 1710 0 9);大连大学科学基金资助项目

摘　　要：The effect of changing the amount of externally added cobalt(Ⅱ) serine, an organic metal compound, and the pH value of the enzyme solution on the interaction of copper zinc superoxide dismutase (Cu 2Zn 2SOD) with cobalt(Ⅱ) serine was studied by means of visible spectroscopy, inductively coupled plasma atomic emission spectrometry and measurement of enzyme activity. It was found that in aqueous solution, there exists a direct interaction of the metal ions of the active center in Cu 2Zn 2SOD with cobalt(Ⅱ) serine. As a result, part of the metal ions in the metalloenzyme were replaced by Co ion to form Co(Ⅱ) substituted derivatives of SOD and the catalytic activity of the enzyme decreased.The effect of changing the amount of externally added cobalt(Ⅱ) serine, an organic metal compound, and the pH value of the enzyme solution on the interaction of copper zinc superoxide dismutase (Cu 2Zn 2SOD) with cobalt(Ⅱ) serine was studied by means of visible spectroscopy, inductively coupled plasma atomic emission spectrometry and measurement of enzyme activity. It was found that in aqueous solution, there exists a direct interaction of the metal ions of the active center in Cu 2Zn 2SOD with cobalt(Ⅱ) serine. As a result, part of the metal ions in the metalloenzyme were replaced by Co ion to form Co(Ⅱ) substituted derivatives of SOD and the catalytic activity of the enzyme decreased.

关 键 词：铜锌 超氧化物歧化酶 丝氨酸 钴 相互作用 核磁共振谱 光谱 配合物 酶活性 

分 类 号：O643.3[理学—物理化学]