高效液相色谱法快速测定抗真菌制剂M18中的吩嗪-1-羧酸  

Determination of Phenazine-1-Carboxylic Acid in Anti-Fungal Agent M18 by High Performance Liquid Chromatography

作　　者：朱栋华[1] 祝新德[1] 许煜泉[1] 

机构地区：[1]上海交通大学生命科学技术学院,上海200240

出　　处：《色谱》2001年第6期558-559,共2页Chinese Journal of Chromatography

基　　金：上海市科学发展基金科技攻关项目资助 ( 995 4130 2 8)

摘　　要：建立了测定抗真菌制剂M18中吩嗪 1 羧酸的反相高效液相色谱方法。流动相为甲醇 5mmol/L磷酸缓冲液(pH 5 0 ) (体积比为 6 0∶40 ) ,流速为 1mL/min ,检测波长为 2 48nm ,线性范围是 5 0mg/L～ 5 0 0mg/L ,检测限是 30mg/L ,回收率为 97 5 3% ,RSD为 1 5 %。该方法具有快速、简便、灵敏。A reversed phase HPLC method for the determination of phenazine 1 carboxylic acid (PCA) in anti fungal agent M18 is established The mobile phase was a mixture of MeOH 5 mmol/L phosphate buffer (pH 5 0) (60∶40,volume ratio) The flow rate was 1 0 mL/min, and the detection wavelength was 248 nm The linear range and detectable limit were 50 mg/L 500 mg/L and 30 mg/L respectively The recovery was 97 53% and RSD was 1 5% The method of PCA extraction and detection has proven to be much faster, simpler, more sensitive, accurate and reproducible than those reported already The assay results can be used as a very important criterion for large scale production

关 键 词：高效液相色谱法 吩嗪-1-羧酸 抗真菌制剂M18 测定 分析 

分 类 号：TQ455.4[化学工程—农药化工]