鲨鳍软骨粘多糖的提取  

EXTRACTION OF MUCOPOLYSACCHARIDE FROM SHARK FIN CARTILAGE

作　　者：肖凯军[1] 郭祀远[1] 李琳[1] 蔡妙颜[1] 官家伦[1] 

机构地区：[1]华南理工大学食品与生物工程学院轻化工研究所,广东广州510640

出　　处：《化工学报》2001年第8期726-729,共4页CIESC Journal

基　　金：国家教育部科学技术重点项目 (教财司 [1999] 2 84);国家自然科学基金资助项目 (No .2 0 0 0 60 0 5 )&&

摘　　要：The effects of extraction time,alkaline concentration,and the addition of cartilage on the extraction of mucopolysaccharides from shark fin cartilage digested with NaOH were investigated.The main components of mucopolysaccharides during the degrading process were analyzed by cellulose polyacetate strip electrophoresis and agarose gel electrophoresis.The results showed that the extraction of mucopolysaccharides was increased with the increase of extraction time,alkaline concentration and the addition of cartilage.According to the different electrophoretic migrations,the preparative mucopolysaccharides composed of different polysaccharides with electronegativity can be divided into two parts,the chondroitin sulfate with higher migration and the other with lower migration.The lower part is unstable and easily degraded with alkaline solution.The effects of extraction time,alkaline concentration,and the addition of cartilage on the extraction of mucopolysaccharides from shark fin cartilage digested with NaOH were investigated.The main components of mucopolysaccharides during the degrading process were analyzed by cellulose polyacetate strip electrophoresis and agarose gel electrophoresis.The results showed that the extraction of mucopolysaccharides was increased with the increase of extraction time,alkaline concentration and the addition of cartilage.According to the different electrophoretic migrations,the preparative mucopolysaccharides composed of different polysaccharides with electronegativity can be divided into two parts,the chondroitin sulfate with higher migration and the other with lower migration.The lower part is unstable and easily degraded with alkaline solution.

关 键 词：粘多糖 鲨鳍软骨 提取 电泳 分析 碱降解法 

分 类 号：TQ464.1[化学工程—制药化工]