灰化法磷测定中不同灰化助剂的效果比较  

A comparison of efficacy of different auxiliaries used in determination of phosphorus by ashing method

作　　者：周毅[1] 张福绥[1] 杨红生[1] 马锡年[1] 

机构地区：[1]中国科学院海洋研究所,青岛266071

出　　处：《分析试验室》2001年第6期58-61,共4页Chinese Journal of Analysis Laboratory

基　　金：国家重点基础研究规划项目G19990 12 0 12;国家自然科学基金项目 (30 10 0 139;30 170 742 );国家重点科技项目 (攻关项目 ) 96 92 2 0 2 0 4

摘　　要：以磷酸二氢钠和磷酸氢二钠与脱氧腺苷三磷酸 (dATP)的混合溶液为磷标样 ,比较了MgSO4,Mg(NO3) 2 和MgCl2 等灰化助剂的作用效果。结果发现 ,磷标样中加入MgSO4经 5 0 0℃灼烧后用 0 .2mol/LHCl于 80℃浸提残渣 0 .5h不能使磷全部回收 ;而MgCl2 、Mg(NO3) 2 、MgAc2 、CaCl2 等助剂 ,却都能使灼烧后的磷完全回收。在采用MgSO4高温灰化法分析海水中的颗粒磷和总磷或有机体中的磷时 ,应当用MgCl2 作为灰化助剂取代MgSO4。尽管Mg(NO3) 2 也是高效的灰化辅助剂 。A comparison of efficacy of different auxiliaries used in determination of phosphorus by ashing method with mixtures of NaH 2PO 4, NaH 2PO 4 and dATP as standard phosphorus samples was made. Phosphorus could not be completely recovered when standard phosphorus ashing with MgSO 4 as an auxiliary. In contrast, auxiliaries of MgCl 2, Mg(NO 3) 2, MgAc 2 and CaCl 2 could make the phosphorus to be recovered completely. We suggest that MgCl 2 rather than MgSO 4, which is usually used, should be used as ashing auxiliary in the phosphorus determination with ashing method.

关 键 词：磷 灰化法 测定 灰化助剂 标准确磷化合物 生物样品 分析 

分 类 号：Q584[生物学—生物化学]