猪传染性胃肠炎病毒纤突糖蛋白N端基因的克隆  

Clone of S gene N terminal of transmissible gastroenteritis coronavirus

作　　者：曹军平[1] 朱爱萍[1] 肖作焕[1] 郑新民[1] 

机构地区：[1]湖北省农业科学院生物技术研究所,武汉430064

出　　处：《湖北农业科学》2001年第5期71-73,共3页Hubei Agricultural Sciences

基　　金：湖北省自然科学基金资助

摘　　要：以RT -PCR方法扩增出TGEV纤突糖蛋白 (S)N端基因片段 ,它包含D、C两个抗原位点 ,长 1 2kb ,平端连接方法将其与质粒PUC18的ECORⅠ多克隆位点相连 ,电转化DH5α 。Transmissible gastroenteritis is an economically important disease in China swine herds The disease produces illness and death losses in young piglet and export markets are closed to breeding stock from TGEV positive herds Molecular techniques were developed to rapidly identify the TGEV The techniques developed are important aids to diagnostic laboratoried and university researchers as a first step in generating a control plan for TGEV disease outbreaks and tracking the spread of the virus N terminal of S gene was cloned into plasmid PUC18 in E coli DH5 α by blunt ligation

关 键 词：猪 传染性胃肠炎病毒 S基因 克隆 纤突糖蛋白N 

分 类 号：S858.286.4[农业科学—临床兽医学]