人类补体第八成份B基因TaqI限制性片段长度多态性研究  

A STUDY OF TaqI RFLP FOR HUMAN COMPLEM ENT COMPONENT C8B GENE

作　　者：张林[1] 李荣华[1] 辛军平[1] 陈国弟[1] 

机构地区：[1]华西医科大学法医学院物证教研究,成都610041

出　　处：《刑事技术》2001年第6期25-26,共2页Forensic Science and Technology

摘　　要：采用补体第八成份B基因(C8B)11号内含子特异性PCR扩增技术和直接TaqI消化处理,检测了121份中国成都地区汉族群体的DNA样本的C8BTaqI限制性片段长度多态性,首次证实了C8BTaqRFLP在中国群体的分布,其基因频率分别为C8BTaqI2.6*=0.4463和C8BTaq1.8,0.8*=0.5537。经计算其表现型频率分布符合Hardy-Weinberg定律,并与德国群体在该基因座的分布进行了比较。Abstrat:The enzyme TaqI restriction fragmen t length polymorphism(RFLP)of human eighth complement B gene(C8B)was studied in 121unrelated Chinese individuals living in Chengdu by using a special PCR -RFLP method.The gene frequencies were estimated as C8B TaqI 2.6*=0.4463and C8B TaqI 1.8,0.8*=0.5537respectively.The observed numbers are in good agreeme nt with Hardy -Weinberg expectation.A comparison of the C8B TaqI RFLP allele frequences indicated that it is different between the Chinese and Germany population(p <0.005)

关 键 词：补体第八成份 限制性片段长度多态性 TAPI 人类 法医学 个人识别 B基因 亲子鉴定 

分 类 号：D919.4[医药卫生—法医学]