固真方对UMR106成骨样细胞蛋白激酶活性的影响  

Effect of Chinese Herb Vitality Reinforcing Formula on Protein Kinase Activity in Rat Osteoblast like UMR 106 Cells

作　　者：张学礼[1] 顾文聪[1] 杜国光[2] 葛韵琴[2] 李平风[2] 

机构地区：[1]上海中医药大学生化教研室,上海200032 [2]北京医科大学生化和分子生物学系,北京100083

出　　处：《中国中医基础医学杂志》2001年第11期23-25,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目 (No.39770 931)

摘　　要：探讨中药固真方对细胞信号转导体系中蛋白激酶活性的影响。结果 :表明经固真方处理的大鼠UMR10 6成骨样细胞的蛋白激酶A、蛋白激酶C以及酪氨酸蛋白激酶活性均有增高 ,分别为对照组的137 5 %、362 4 %及 2 0 2 6% (P <0 0 1或P <0 0 5 )。提示 :固真方可活跃和增进细胞的信号转导 ,这可能是其促细胞增殖的一种机制。Objective:To study the effect of Chinese herb Vitality Reinforcing Formula on protein kinase activity of cellular signaling transduction system.Methods:UMR 106 cells were cultured in MEM,divided into VRF group and control group.After culture,protein kinse samples were prepared,assayed for protein kinase A,protein kinase C and Tyrosine phosphokinase.Results:VRF treatment can increase PKA、PKC and TPK activity of 137 5%、362 4% and 202 6% respectively in UMR 106 cells.Conclusion:It was suggested that VRF does play important roles in activating cellular signaling transduction in UMR 106 cells.

关 键 词：固真方 蛋白激酶A 蛋白激酶C 酪氨酶蛋白激酶 

分 类 号：R289.5[医药卫生—方剂学]