芦荟大黄素与血清白蛋白的相互作用  

The Interaction of Serum Albumin with Aloe-emodin

作　　者：王敬政[1] 贺吉香[1] 江崇球[1] 

机构地区：[1]山东师范大学化学系,济南250014

出　　处：《分析化学》2001年第7期782-784,共3页Chinese Journal of Analytical Chemistry

基　　金：山东自然科学基金资助项目

摘　　要：利用荧光光度法研究了中药有效成分芦荟大黄素与血清白蛋白的相互作用机制 ,求得两者的形成常数 ,讨论了某些金属离子对其形成常数的影响 ,并根据热力学常数确定了它们之间的作用力类型 ,利用F rster非辐射能量转移技术求出了两者的结合位置。In this paper, we have studied the interaction of serum albumin (BSA and HSA) with Aloe emodin by fluorescence quenching method. The binding constants were obtained at different temperatures. We have also discussed the binding constants in the presence of various metal ions. According to the thermodynamic parameters, the main sorts of binding force have been obtained. The results showed that. the mechanism of quenching belonged to static quenching and the main sorts of binding force are static electricity gravitation and Van der Waals force.

关 键 词：芦荟大黄素 血清白蛋白 荧光猝灭法 植物药 作用机制 相互作用 药理 

分 类 号：O657.3[理学—分析化学]