虫荧光素酶报告基因用于二噁英类化学物质的检测  

Detection of Dioxin-type Chemicals by Luciferase Reporter Gene

作　　者：张志仁[1] 徐顺清[1] 周宜开[1] 任恕[1] 刘志伟[1] 

机构地区：[1]华中科技大学同济医学院环境医学研究所,武汉430030

出　　处：《分析化学》2001年第7期825-827,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助课题 (No .2 98770 2 0 )

摘　　要：虫萤光素酶报告基因检测二英类化学物质与传统的EROD法比较 ,灵敏度及线性范围均优于EROD法 ,并且检测时间缩短 ,操作简便。Detection of dioxins like chemicals with luciferase reporter gene can screen lost of samples and quickly semi quantitate. It was compared with the traditional ethoxyresorufin O deethylase (EROD) method. the result suggested that the luciferase induction was 3 fold more sensitive than EROD induction, the detection time was shorter for 48 hours and the detection procedure was also simpler.

关 键 词：二恶英 虫荧光素酶 报告基因 环境污染物 环境监测 分析 生物监测 

分 类 号：O656[理学—分析化学]