正常与重金属铅注射的兔脑蛋白质双向电泳图谱比较与鉴定  

Comparison and Identification of Rabbit Brain Proteins from the Normal and PbCl_2 Injected Rabbit by High-resolution Two-dimensional Gel Electrophoresis and Mass Spectrometry

作　　者：赵锐[1,2] 纪建国[1] 郝守进[1] 陈倩[1] 袁洪生[1] 管华诗[2] 茹炳根[1] 

机构地区：[1]北京大学生命科学学院,蛋白质工程及植物基因工程国家重点实验室,北京100871 [2]青岛海洋大学海洋药物与食品研究所,青岛266003

出　　处：《生物化学与生物物理进展》2001年第6期874-879,共6页Progress In Biochemistry and Biophysics

摘　　要：重金属污染对人类健康的威胁日益受到关注 ,为了了解大量重金属摄入对脑蛋白质的影响 ,对比研究了正常兔脑组织蛋白质与重金属铅腹腔注射 2周后的兔脑组织在蛋白质双向电泳图谱中的差异 ,分析重金属注射对脑蛋白质表达的可能影响 .通过对脑组织蛋白质的提取 ,分离出水溶性的蛋白质组分 ,经双向电泳图谱比较正常与注射重金属铅的兔子在脑蛋白质表达上的差异 ,其中 3个蛋白质斑点经提取 ,反相高效液相色谱 (RP HPLC)分离 ,基质辅助激光解析电离质谱 (MALDI TOFMS)确定了分子质量 ,并利用肽质量指纹图谱检索数据库确定蛋白质的归属 .The pollution of heavy metals may affect the human health, in order to study the affect of heavy metals to the brain, the normal alive rabbits and the rabbits which were injected with PbCl2 for 2 weeks were autopsied, the brain proteins were extracted and separated by gel filtration, and further analyzed by 2-D PAGE, More than one hundred water-soluble proteins of rabbit brain were visualized by Coomassie Brilliant Blue staining. The apparent molecular masses of most water-soluble proteins from rabbit brain were about 6 similar to 30 ku, pI was about 4 similar to9. After matched with normal alive rabbit brain protein spots separated by 2D-PAGE, there obviously exists many differences displayed and unmatched protein spots in the 2-D PAGE maps of Pb2+ induced rabbit brain. 3 spots were extracted from the 2D-PAGE and detected by the MALDI-TOF-MS and ESI-MS, The experiments show that rabbit brain proteins would be affected by Pb2+ injection.

关 键 词：蛋白质组学 双向电泳 基质辅助激光解析电离质谱 电喷雾质谱 脑蛋白 图谱比较 

分 类 号：Q51[生物学—生物化学]