苦瓜谷胱甘肽磷脂氢过氧化物酶cDNA的克隆及其特征分析  

Cloning and Characterization of a Phospholipid Hydroperoxide Glutathione Peroxidase Gene from Momordica charantia

作　　者：李文君[1] 刘进元[1] 赵南明[1] 

机构地区：[1]清华大学生物科学与技术系,北京100084

出　　处：《生物化学与生物物理进展》2001年第6期908-911,共4页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金 (39770 0 78;30 1 70 0 80 );国家重点基础研究专项 (G1 9980 1 0 1 0 0 );云南省校合作基金 (98YQ0 1 0 );国家转基因植物研究专项 (J99 A 0 4 1 )资助项目~~

摘　　要：根据谷胱甘肽磷脂氢过氧化物酶 (PHGPX)氨基酸序列中高度保守的区段设计引物 ,采用RACE PCR从苦瓜中克隆到一个全长 92 7bp的cDNA片段 .DNA序列的数据库分析比较表明 ,该cDNA编码 16 7个氨基酸 ,含有动植物PHGPX的特征结构 ,是一个新发现的苦瓜PHGPX基因 (mocPHGPX) .RNA印迹结果显示 ,该基因在苦瓜幼苗的根中表达相对较弱 ,茎的信号较强 ,叶中最强 .Using RACE-PCR technique, a full-length cDNA was cloned from Momordica charantia and named mocPHGPX. The entire mocPHGPX cDNA (AF346906) is 927 bp, and encodes 167 amino acids. The derived amino acid sequence data showed that this protein contains the typical ( high conservative) structure of phospholipid hydroperoxide glutathione peroxidase (PHGPX) previously found in animals and plants. Northern blot analysis displayed that the corresponding transcript is abundant in the leaves and stems but lower in the roots. These results will be helpful to make a thorough investigation of the function of plant PHGPX and to understand the anti-oxidative systems of plants in an all-round way.

关 键 词：苦瓜 CDNA 克隆 序列特征 谷胱甘肽磷脂氢过氧化物酶 植物 

分 类 号：Q943.2[生物学—植物学] S642.5[农业科学—蔬菜学]