剪切力对单核细胞趋化蛋白-1的影响  

Fluid Shear Stress Regulates the Synthesis and Secretion of Monocyte Chemotactic Protein-1

作　　者：于红梅[1] 曾衍钧[1] 胡金麟[2] 李彩霞[1] 

机构地区：[1]北京工业大学生物医学工程中心,北京100022 [2]中国人民解放军总医院微循环研究室,北京100853

出　　处：《生物化学与生物物理进展》2001年第6期912-915,共4页Progress In Biochemistry and Biophysics

基　　金：北京市自然科学基金资助项目 (3982 0 0 5)~~

摘　　要：单核细胞趋化蛋白 1(MCP 1)能趋化单核细胞在内皮细胞下聚集 ,是动脉粥样硬化最早期的病理改变之一 .从生物力学的角度对体外培养的人脐静脉内皮细胞 (HUVEC)合成和分泌MCP 1的规律作了研究 .通过流动小室 ,HUVEC给予 0 4,1 0 ,2 0N/m2 的剪应力 ,运用免疫组化 ,图象处理及ELISA方法测出不同时间胞浆及灌流液中MCP 1的含量 ,结果表明HUVEC合成和分泌MCP 1是随剪切力和时间变化而变化的 .Monocyte chemotactic protein-1 (MCP-1) is a potent special chemoattractant capable of recruiting monocytes into the subendothelium. In order to study the patterns of fluid shear stress on induction of MCP-1 secretion in cultured human umbilic vein endothelial cells (HUVEC), the relationship of fluid shear stress and the secretion of MCP-1 was examed, cultured HUVEC were subjected to controlled levels of shear stress (0.4, 1.0, 2.0 N/m(2)) in a parallel plate flow chamber. MCP-1 in HUVEC of different periods was measured by immunohistochemistry method and image analysis; MCP-1 in perfusion is measured by ELISA. The results of the experiment demonstrated the secretion of MCP-1 regulated by shear stress was time- and force-dependent. The accumulated level of MCP-1 in HUVEC under lower shear stress (0.4 N/m(2)) for 4 similar to5 h was 3-fold compared with that for static cells. When the shear stress lasted to 6 h, the secretion of MCP-1 was reduced to normal levels and could not be increased. Such research provides data for understanding the mechanism of the contribution of hemodynamic forces to atherosclerosis.

关 键 词：剪切力 脐静脉内皮细胞 单核细胞趋化蛋白-1 MCP-1 动脉粥样硬化 

分 类 号：R543.502[医药卫生—心血管疾病] R318.01[医药卫生—内科学]