小麦-大赖草易位系的RFLP分析  

RFLP Analysis of Wheat- L. racemosus Translocation Lines

作　　者：王秀娥[1] 陈佩度[1] 周波[1] 袁建华[1] 刘文轩[1] BikramS Gill 刘大钧[1] 

机构地区：[1]南京农业大学农业部作物细胞遗传重点开放实验室,南京210095 [2]DepartmentofPlantPathology

出　　处：《Acta Genetica Sinica》2001年第12期1142-1150,T001,共10页

基　　金：国家高新技术研究发展计划 ( 863计划 ) ( 1 0 1 -0 2 -0 1 -0 2 );美国McKnight基金作物研究计划;江苏省自然科学基金 (BK970 90 );高校博士点基金 ( 970 2 0 5 )

摘　　要：利用辐射、花药培养及杀配子基因效应已创制出一系列小麦 -大赖草易位系。为在其中找出可能的纯合易位系、明确易位所涉及的相关染色体以及易位断裂点的确切位置 ,利用了已被定位于小麦 7个部分同源群染色体长、短两臂上的 67个探针进行了RFLP分析 ,结果鉴定出 3个纯合的易位系 :T1BL·7Lr#1S、T4BS·4BL - 7Lr#1S和T6AL·7Lr#1S。其中 ,易位系T1BL·7Lr #1S和T6AL·7Lr #1S中染色体 7Lr #1的断裂点位于标记MWG80 8和标记ABG476.1之间 ,而 1B和 6A染色体上的断裂点都在着丝粒附近。易位系T4BS·4BL - 7Lr#1S中染色体 7L #1的断裂点位于标记BCD349和标记CDO5 95之间 ,4B染色体断裂点则位于标记CDO5 4 1和标记PSR1 64之间的长臂上。A number of wheat L. racemosus translocation lines were developed by irradiation, pollen culture and gametocidal chromosome methods. In order to identify homozygous translocation lines and determine the exact location of the breakpoints involved in the translocations, 67 probes genetically or physically mapped previously on wheat chromosomes belonging to seven homoeologous groups were used for RFLP analysis. Three homozygous translocation lines were identified: T1BL·7Lr#1S, T4BS·4BL 7Lr#1 and T6AL·7Lr#1S. In lines T1BL·7Lr#1S and T6AL·7Lr#1S,the breakpoint of chromosome 7Lr#1 was located in the short arm between the area marked by clone MWG808 and that of ABG476.1, and the breakpoints of chromosomes 1B and 6A were both located near the centromere. In line T4BS·4BL 7Lr#1S, the breakpoint of chromosome 7Lr#1 was located in the short arm between the area marked by clone BCD349 and that of CDO595, the breakpoint of chromosome 4B was located in the long arm between the area marked by clone CDO541 and that of PSR164.

关 键 词：小麦 大赖草 易位系 RFLP分析 

分 类 号：S512.1[农业科学—作物学]