几种药用植物内生真菌抗真菌活性的初步研究  

STUDY ON THE ANTIFUNGAL ACTIVITIES OF ENDOPHYTIC FUNGI ISOLATED FROM SEVERAL PHARMACEUTICAL PLANTS

作　　者：李桂玲[1] 王建锋[1] 黄耀坚[1] 郑忠辉[1] 苏文金[1] 

机构地区：[1]厦门大学生命科学院,厦门361005

出　　处：《微生物学通报》2001年第6期64-68,共5页Microbiology China

基　　金：教育部高等学校博士学科点专项科研基金资助项目 (No .96 0 384 0 2 )

摘　　要：从三尖杉 ,南方红豆杉及香榧中分离出 1 72株内生真菌 ,对其进行抗菌活性检测 ,结果表明共 90株内生真菌对一种或多种植物病原真菌 ,如红色面孢霉 (Neurosporasp .) ,木霉 (Trichodermasp .) ,镰刀菌 (Fusariumsp .)等有抑制作用 ,来自三尖杉、南方红豆杉和香榧的抗菌活性菌株比例分别为 40 %,5 4 2 %及 5 7 1 %。其中平板抑菌圈直径大于1 5mm的高抗菌株有 3 5株。按Ainsworth等鉴定系统和方法进行鉴定 ,具有抗菌活性的内生真菌主要分布于拟青霉属、镰孢菌属等 1 8个属中。One hundred and seventy-two strains of endophytic fungi were isolated from Taxus mairei,Cephalotaxus fortunei and Torreya grandis cv.merrillia.The result of the antifungal assay shows that ninety strains of the fungi have antagonism against one or more botanical pathogenic fungi,such as Neurospora sp.,Trichoderma sp.,Fusarium sp.etc.The percentage of antifungal strains to tested strains are as follows:40% Cephalotaxus fortunei,54.2% Taxus mairei,57.1% Torreya grandis cv.merrillia.Thirty-five strains have high antifungal activities,and their inhibition zone diameter is at least 15mm.The active endophytic fungi were identified as 18 genera,most of which belong to Paecilomyces and Fusarium etc.

关 键 词：内生真菌 抗真菌作用 药用植物 

分 类 号：Q948.122.3[生物学—植物学]