转bar基因水稻在杂种优势育种中的利用  

APPLICATION OF BAR-TRANSGENIC RICE IN BREEDING FOR HETEROSIS

作　　者：段发平[1] 黎垣庆[1] 梁承邺[1] 

机构地区：[1]中国科学院华南植物研究所,广东广州510650

出　　处：《热带亚热带植物学报》2001年第4期329-334,共6页Journal of Tropical and Subtropical Botany

基　　金：国家转基因植物研究与产业化开发项目基金资助

摘　　要：以3个美国转bar基因水稻抗除草剂品种BengalHu-10、CypressPB-6和Gulfmont为父本,分别与三系、两系不育系及人工去雄恢复系杂交。考种结果发现:这3个抗性亲本所配组合杂种优势不明显。通过杂交将抗除草剂亲本中的bar基因转移到常规恢复系,已经培育出3个抗除草剂恢复系明恢63-B、测64-B和特青-B;且已选配出5个抗性杂交组合,它们能保持原组合的产量水平。还讨论了水稻杂种优势利用面临的问题和转bar基因水稻抗性亲本在杂种优势上的应用前景。Threebar-transgenicrices,BengalHu-10,CypressPB-6,andGulfmontas maleparents,were crossedwith6malesterilelines:Zhenshan97A,Bo A,Pei-ai64S,Nanxiang95S,Xi217SandGD1-S.Eightrestorersemasculatedwerecrossedwiththethreeherbicide-resistantsources.Forty-eightF 1 hybrids wereobtainedtotally.The resultsindicatedthatheterosisin theseF 1 hybridswere not obvious.By transferringherbicide(Liberty)-resistantbar gene into conventionalrestorerlines,3restorerlines(Minghui63-B,Ce64-B,andTeqing-B)weredevelopedviacrossing,and5resistantcombinations,i.e Zhenshan97A/Minghui63-B(B 2 F 1 -B),Zhenshan97A/Ce64-B(B 2 F 2 -G),Zhenshan97A/Ce64-B(B 3 F 1 -C),Pei-ai64S/Teqing-B(B 1 F 2 -C),andPei-ai64S/Teqing-B(B 2 F 1 -B),hadbeenbred,whichretainedthesame yieldinglevelof theiroriginalcombinations.Theproblemsof heterosisapplication,andtheprospectof bar-transgenicriceparentsinbreedingforheterosisarediscussed.

关 键 词：转BAR基因水稻 杂种优势 雄性不育 除草剂 育种 

分 类 号：S511.035.1[农业科学—作物学]