草酰乙酸和苹果酸对菠菜叶片和完整叶绿体光合作用的影响  

Effects of Oxaloacetate and Malate on Photosynthesis in Leaves and in Intact Chloroplasts from Spinach

作　　者：陈根云[1] 叶济宇[1] 

机构地区：[1]中国科学院上海植物生理研究所,上海200032

出　　处：《植物生理学报（0257-4829)》2001年第6期478-482,共5页Acta Phytophysiologica Sinica

基　　金：国家重点基础研究发展规划 (批准号 :G19980 10 10 0 )资助项目

摘　　要：观测了OAA和MA对菠菜叶片和完整叶绿体光合作用的影响。结果显示 ,当叶片切块在 2 0μmol/L 的OAA存在时 ,其叶片的光合放氧速率增加了 89% ,经OAA处理的离体完整叶绿体的光合放氧速率增加了 72 % ;当反应体系中存在有较高浓度的NaHCO3时 ,OAA的作用不明显。叶片经 2 0 μmol/L的MA处理后 ,叶片光合放氧速率比对照高 12 7%。用CO2 分析仪观测了处理后叶片的净光合速率 (Pn) ,结果显示 ,OAA和MA处理后的叶片Pn值分别是对照的 117%和 111%。对在C3植物中建立C4微循环系统来提高光合作用效率的可能性进行了讨论。Effects of oxaloacetate(OAA) and malate(MA) on photosynthesis in leaves and intact chloroplasts from spinach were measured: first, the rate of photosynthetic oxygen evolution in leaves increased by 72% after 20 μmol/L OAA treatment(Fig.1A), and that in intact chloroplasts increased by 78%(Fig.5), but no obvious effect of OAA was observed when NaHCO 3 0.5 mmol/L was present in the assay mixture(Fig.1B). When leaves and intact chloroplasts were treated by MA at a concentration of 20 μmol/L, the rate of photosynthetic oxygen evolution increased by 127% (Fig.3)and 18% respectively(Fig.5). Moreover, net photosynthetic rate (Pn) of leaves were measured after OAA and MA treatment at the concentration of 200 μmol/L, the Pn value were increased by 17% and 11% respectively(Fig.4). Finally, it was discussed the possibility to improve the photosynthetic efficiency by building up a micro cyclic system of C 4 dicarboxylic acid in C 3 plants.

关 键 词：C4循环 草酰乙酸 苹果酸 C3植物 菠菜叶片 叶绿体 

分 类 号：Q945.78[生物学—植物学]