光敏雄性不育谷子 683小孢子败育途径观察  

Observation on the microspore abortion of photoperiod-sensitive male sterile miliet (Setaria italica) 683

作　　者：孙春昀[1] 范昌发[2] 崔贵梅 孙毅 张福耀 何军贤[3] 郭骁才[4] 

机构地区：[1]山西省农业生物技术研究中心,太原030031 [2]西北大学生命科学学院,西安710069 [3]四川大学生命科学学院,成都610064 [4]中国科学院成都生物研究所,成都610041

出　　处：《西北植物学报》2001年第6期1117-1122,T001-T002,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：山西省财政厅科技支农项目 ( 980 0 3);山西省留学回国人员项目 ( 981 0 1 5 )资助;中国科学院生物科学与技术研究特别支持费课题 ( STZ-1 -1 6 )

摘　　要：对光敏雄性不育谷子 (Setaria italica) 6 83在长日 (>1 5 h)和短日 (=1 0 h)下小孢子的发育途径、花药中花粉总数及游离花粉总数作了观察和统计。发现长日条件下 ,小孢子败育主要发生在造孢细胞和花粉母细胞时期 ,此时 ,有 6 0 %～ 80 %的药室的造胞细胞和花粉母细胞严重收缩 ,细胞结构破坏 ,演变成着色极深的“异常黑块”(埃氏苏木精染色 )。每花药中花粉平均数 ,长日条件下为 6 6 .84,短日照下为 1 1 1 .86 ,差异显著。每视野中的游离花粉平均数 ,长日条件下为 1 3 .73 ,而在短日条件下为 2 0 .1。研究对“异常黑块”的出现与花粉总数大幅度降低的关系作了分析 ,并讨论了“异常黑块”The developmental process of microsporogenesis,the number of pollens in anther and the number of free pollens of the photoperiod sensitive male sterile Millet under long day cycles (LD,>15 h) and short day cycles (SD,=10 h) were investigated.Some special abortive types were detected in the sporogenous cells or microspore mother cells,60%~80% of sporogenous cells or microspore mother cells shrank,and their structure was destroyed,they developed into abnormal black clumps at last.The average number of pollens per anther and free pollens per field of version under long day cycles and short day cycles were 66.84,13.73 (under LD) and 111.86,20.1 (under SD) respectively.The relationship between the appearance of abnormal black clumps and decline of pollen number was analyzed.The results suggested that the appearance of abnormal black clump might cause microspore abortion.

关 键 词：光敏雄性不育 谷子 造孢细胞 小孢子母细胞 败育 

分 类 号：S515[农业科学—作物学]