玉米离体根尖的多层滤纸床液体静止培养方法  

Establishment of liquid culture method for in vitro maize root tip with filter-papers-bed and motionless

作　　者：张一[1] 段留生[1] 翟志席[1] 何钟佩[1] 

机构地区：[1]中国农业大学作物化学控制研究中心,北京100094

出　　处：《西北植物学报》2001年第6期1188-1193,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金资助项目 ( 39870 4 83)

摘　　要：设计建立了适于玉米根尖离体培养的多层滤纸床液体静止培养方法 ,培养的适宜体系为 :1 /4MS大量元素改良 +1 /2 MS微量元素 +IBA0 .1～ 0 .3 mg/L,黑暗培养。该方法避免了传统液体培养通气状况不良的问题 ,玉米根的生长速度可达到 1～ 2 cm/d,分支和生长正常。该方法在控制条件下快速繁殖根系 ,成本低廉 ,简便易行 。A new liquid culture using filter papers with motionless for corn root apical were established in this paper.The suitable culture medium included 1/4 enormous elements of MS,1/2 trace elements of MS and IBA 0.1~0.3 mg/L,and the roots should be grown under dark.The system gave the corn roots better aerate condition and made the roots grew faster than traditional liquid culture.Corn root grew by the rate of 1~2 cm/d,while developed and branched normally.The new method made in vitro cloning of roots fast and simply,and was proved to be the ideal experimental system for study on development and physiology of plant roots.

关 键 词：玉米 根尖培养 根系 离体培养 多层滤纸床液体静止培养 

分 类 号：Q943.1[生物学—植物学] S336[农业科学—作物遗传育种]