益气化瘀中药对脑缺血再灌注大鼠白细胞粘附及内皮素的影响  

Effects of TCM Drugs for Benefiting Qi and Resolving Blood Stasis on Leukocyte Adhesion and the level of Endothelin-1 in the Rats Undergone Cerebral Ischemia and Reperfusion

作　　者：郭改会[1] 胡清华[1] 董鸿瑞[1] 庄逢源[1] 傅仁杰[1] 

机构地区：[1]中日友好医院,北京100029

出　　处：《北京中医药大学学报》2001年第5期34-37,共4页Journal of Beijing University of Traditional Chinese Medicine

摘　　要：观察了益气化瘀中药通络熄风注射液对脑缺血再灌注模型大鼠软脑膜微循环中白细胞粘附及血浆和脑组织内皮素含量的影响。结果 :脑缺血再灌注后微循环的血流速度明显加快 ,而后对照组动物可见血流速度又逐渐减慢 ,白细胞标记显示这种流速减慢的原因是白细胞在微血管内皮细胞表面的粘附及微静脉内微小血栓的形成 ,从而造成微循环的机械性阻塞。内皮素 (ET)测定结果显示对照组血浆及脑组织的ET 1水平明显增高。较大剂量的通络熄风注射液能够明显抑制白细胞与血管内皮细胞的粘附 ,抑制微小血栓的形成 ,并能降低血浆和脑组织的ET 1的含量 ,改善脑缺血再灌注后的微循环障碍 ,从而对脑缺血再灌注损伤起到保护作用。The effects of Tongluoxifeng Injection (TI) on leukocyte adhesion in piamatral microcirculation and the level of endothelin 1 in the plasma and brain in the rat model of cerebral ischemia and reperfusion were investigated. In the model control group, the blood flow speed of microcirculation became obviously higher at first and then gradually lower, and the leukocyte labeling test revealed that it was because of the leukocyte adhesion on the surface of micrangium endothelial cells and the formation of minute thrombus in venulae, hence causing the mechanical obstruction of microcirculation; furthermore, the endothelin 1 determination revealed that the level of endothelin 1 in the plasma and brain became obviously higher. It was found that a relatively large dose of TI could markedly obstruct leukocyte adhesion on the surface of micrangium endothelial cells, inhibit the formation of minute thrombus, lower the level of endothelin 1 in the plasma and brain, remove the obstruction caused by ischemia and reperfusion in microcirculation, hence exerting protective effects against the lesions due to ischemia and reperfusion.

关 键 词：益气化瘀 通络熄风注射液 脑缺血再灌注 白细胞粘附 内皮素 中药 

分 类 号：R285.5[医药卫生—中药学]