疣粒野生稻胚性愈伤组织诱导及分化成苗研究  

Studies on Inducement of Embryogenic Callus from Oryza meyeriana and Plantlets Regeneration

作　　者：秦发兰[1] 陈葆棠[1] 朱永生[1] 林兴华[1] 张端品[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《中国农业科学》2001年第5期560-563,共4页Scientia Agricultura Sinica

基　　金：国家"九五"生物技术攻关计划项目 ( 96-C0 1-0 1-0 6)

摘　　要：疣粒野生稻是优异的稻种资源。目前用常规方法及分子标记技术利用其有利基因还非常困难 ,体细胞杂交技术是一条比较可行的途径 ,但疣粒野生稻是一种典型的难培养植物 ,体细胞杂交的前期研究非常重要。本研究以疣粒野生稻的成熟种子为外植体 ,采用不同的培养基诱导愈伤组织 ,并对愈伤组织的状态进行改造 ,结果表明 ,NB2 培养基最适宜于诱导愈伤 ,添加 0 .1% PG及 0 .2 m g/ L KT有利于愈伤状态的改造 ;愈伤分化时采用 NBK2 培养基效果好 ,绿苗分化率高 ;采用 A2 及 NB2 液体培养基振荡培养 。Oryza meyeriana is one of excellent germplasms. At present, it is difficult to transfer its genes by conventional methods and molecular technologies. However, somatic fusion is one of feasible approaches. The preliminary research of somatic fusion is crucial because O. meyeriana is recalcitrant to regeneration. High quality embryogenic calli and green plantlets were obtained from mature seeds of wild rice Oryza meyeriana. The result indicated that NB medium was excellent for calli induction when 2mg/L 2,4 D, 0.1%PG and 0.2mg/L KT were added. And NB medium with 2mg/L KT was also excellent for high ratio of green plantlet differentiation. High quality embryogenic cell suspension lines were obtained with AA 2 and NB 2 liquid media by rotating culture.

关 键 词：疣粒野生稻 胚性愈伤组织 细胞悬浮系 绿苗分化 成苗 

分 类 号：S511.9[农业科学—作物学]