渗透胁迫下芦苇胚性愈伤组织中脯氨酸含量的变化及外源ABA的影响  

Changes of Free Proline Contents of the Calluses from Reed Ecotypes to the Osmotic Stress and Exogenous ABA

作　　者：陈龙 刘怀攀 吴诗光 崔素霞[2] 张承烈[2] 

机构地区：[1]周口师范高等专科学校生物系,河南周口466000 [2]兰州大学生命科学学院

出　　处：《种子》2001年第5期11-12,共2页Seed

基　　金：国家自然科学基金资助项目 (编号 :79570 0 75)部分内容

摘　　要：研究了 PEG- 6 0 0 0胁迫下两种生态型芦苇胚性愈伤组织游离脯氨酸含量的变化及外源 ABA对其变化的影响。结果表明 :在 10 %～ 30 % PEG的胁迫下脯氨酸迅速积累 ,沙生芦苇胚性愈伤组织 (SREC)的积累量明显高于水生芦苇胚性愈伤组织 (WREC)。施加 10μmol/ L ABA后 WREC的脯氨酸含量大幅度升高 ,但对 SREC的影响较小。The changes of free proline contents of the calluses from two kinds of reed ecotypes have been detected. The results were as below; There was a rapid proline accumulation in both of the embryogenic calluses of sand reed (SREC) and water reed (WREC) to the PEG osmotic stress treatment from 10% to 30%, and the proline content of SREC was much higher than that of SREC. It also showed that response of WREC to exogenous ABA (10μmol/L) was much higher than of SREC in the aspect of prolineaccumulation.

关 键 词：生态型 芦苇 胚性愈伤组织 渗透胁迫 ABA 游离脯氨酸 抗旱性 

分 类 号：S332.4[农业科学—作物遗传育种] Q948.112.3[农业科学—农艺学]