衰老时大鼠大脑皮质枕叶神经肽Y阳性神经元的变化  

Changes of Neuropeptide Y-Positive Neurons in Occipital Cortex of Rats in Aging

作　　者：王荣华[1] 李秋明[1] 付宜鸣 张宝东[1] 宋铁军[1] 

机构地区：[1]哈尔滨医科大学组胚教研室,哈尔滨150086 [2]附属第二医院骨外科,哈尔滨150086

出　　处：《解剖科学进展》2001年第4期312-314,共3页Progress of Anatomical Sciences

摘　　要：目的　　研究衰老时神经肽Ｙ（ＮＰＹ）阳性神经元在大鼠大脑皮质枕叶中的变化情况。方法　　选用青年组（１～２个月）、中年组（１１～１２个月）和老年组（２２～２４个月）三组Ｗｉｓｔａｒ大鼠，采用免疫组化ＳＡＢＣ法结合计算机图像分析技术进行研究。结果　　ＮＰＹ阳性细胞属于非锥体形神经元，在枕叶皮质Ⅱ—Ⅵ层均有分布；比较三个年龄组阳性细胞的数量和平均截面积的变化，发现老年组与青年组及中年组相比，两项指标都显著减小（Ｐ＜０．０１），而青年组和中年组相比，两项指标都没有显著性变化（Ｐ＞０．０５）。结论　ＮＰＹ作为神经调节物，可能与大脑皮质枕叶功能的调节有关；随着衰老其阳性神经元的变化可能影响ＮＰＹ发挥正常的生理功能。Objective To study changes of neuropeptide Y-positive neurons in occipital cortex of rats in aging. Methods The infant(1-2months) and the adult(11-12months) and the old group (22-24months) consisted of Wistar rats. Immunohistochemical SABC method combined with image quantitative analysis were used. Results NPY-positive neurons were shown non-pyramidal form, distributed in layers Ⅱ-Ⅵ of cerebral cortex; compared the old group with the infant and the adult, there was a significant decrease(P<0.01) in quantity and average area of positive cells, but compared the infant group with the adult group, there was not significant change (P>0.05).Conclusion As neurotransmitter, NPY was probably related to the regulation function of occipical cortex, with aging changes of NPY-positive neurons influence natural physiological function of NPY.

关 键 词：神经肽Y 大鼠 大脑皮质 衰老 免疫组织化学 

分 类 号：R339.38[医药卫生—人体生理学] R322.81[医药卫生—基础医学]