小毛莨内酯对结核休眠菌感染病人GLS mRNA表达的影响  

Effects of ternatolide on expression granulysin mRNA in the peripheral blood lymphocytes of the patients infected by dormant tuberole bacilli

作　　者：詹莉[1] 戴华成 杨治平[3] 易著文[1] 成诗明 舒平[5] 

机构地区：[1]中南大学湘雅二医院,长沙410011 [2]美国新泽西医学,牙学医学院药分子生物学研究所 [3]湖南农业大学植物科学研究院,410128 [4]湖南省结核病防治所,410006 [5]华中科技大学同济医学院药学院,430030

出　　处：《中国防痨杂志》2001年第5期275-277,共3页Chinese Journal of Antituberculosis

基　　金：国家自然科学基金资助课题 (No .3930 0 139 No .39670 334 No .39970 32 6)

摘　　要：目的　研究小毛莨内酯抗人体结核休眠菌感染的分子免疫学机理。方法　采用PCR方法检测结核病人PBLs中结核菌 16kDα 晶状体同源蛋白DNA阳性的 5 6例患者为本研究对象 ;采用反转录聚合酶链反应半定量 (QC RT PCR)方法 ,测定不同剂量的Tern .对以上病人PBL颗粒裂解肽mRNA表达水平的影响。结果　当Tern .在 10 0mg L ,2 0 0mg L浓度时 ,能诱导结核休眠菌感染病人体外活化的PBLGLSmRNA的表达。结论　Tern .可能通过促进颗粒裂解肽mRNA的表达 ,增强机体CTL杀菌能力 ,达到抗结核休眠菌的作用。Objective To study the effect of ternatolide (Tern.) on expression of granulysin mRNA in the activated peripheral blood lymphocytes (PBLs) of the patients infected dormant tubercle bacilli (DTB). Methods 16kD α crytallin homolog gene of DTB in PBLs of 56 patients was detectd by PCR.Using competitive quantitative reverse transcription polymerase chain reaction (QC RT PCR) analyze granulysin gene expression in the PBLs.Results The dosage of 100mg/L and 200mg/L of ternatolide significantly enhanced the granulysin mRNA experession in PBLs in vitro stimulated phytohemagglutinin (PHA) from the patients with DTB ( P <0.001).Conclusion The molecular mechanism of Tern. against intracellular pathogens might be associated with the inducting highly level on the expression of granulysin mRNA,a anti bacterial peptide in activated human cytotoxic lymphocytes.

关 键 词：小毛莨内酯 颗粒裂解肽 结核休眠菌 18kD α-晶状体同源蛋白 GLS MRNA 

分 类 号：R52[医药卫生—内科学]