用^(111)In标记单克隆抗体和动物分布与显象研究  

LABELLING OF McAb WITH ^(111)In AND ITS BIODISTRIBUTION AND IMAGING STUDIES

作　　者：吴传初 吴永慧[1] 刘元方[1] 吴红[2] 王世真[2] 韩冰[3] 刘成贵[3] 袁玫[3] 赵东海[3] 

机构地区：[1]北京大学技术物理系 [2]北京协和医院核医学科 [3]解放军总医院肿瘤单抗室

出　　处：《核化学与放射化学》1989年第3期156-162,共7页Journal of Nuclear and Radiochemistry

基　　金：中国自然科学基金

摘　　要：研究了用^(111)In通过DTPA环二酸酐标记单克隆抗体(McAb)的各个实验环节,制备了超纯^(111)In,得到了高比活度1.11GBq/mgMcAb、具有免疫反应活性的^(111)In-McAb。对用^(111)In标记的抗CEA McAb C14-C50、抗人结肠癌McAb 2C10和鼠IgG在荷瘤裸鼠模型体内分布及体外显象进行了研究。注射单抗的动物得到清晰的肿瘤显象,而注射普通IsG的动物的肿瘤不显象。The labelling of McAb with 111In through DTPA bicyclic anhydride has been systematically studied. The 111In-labelled McAb with high specific radioactivity, up to 1.11GBq/mg, without loss of immunoreactivity can be obtained by using ultrapure 111In. The biodistribution and imaging of 111In-labelled anti-CEA McAb C14-C50, anti-colon cancer McAb 2C10 and non-specific mouse IgG in nude mice bearing human colon tumors are investigated respectively. Clear tumor localizations are observed on mice injected with 111In-labelled McAbs, whereas no tumor localization appears in contrast of the tumor-bearing-mice injected with non-specific IgG.

关 键 词：单克隆抗体 铟111 标记 免疫显象 

分 类 号：R817.4[医药卫生—影像医学与核医学]