叶绿素缺乏大麦突变体叶绿体结构功能及生化特性的研究  

STUDIES ON THE STRUCTURE AND FUNCTION OF CHLOROPLAST IN A CHLOROPHYLL-LESS BARLEY MUTANT(NYB)

作　　者：林宏辉[1] 何礼[1] 晏婴才[1] 代其林[1] 杜林方[1] 梁厚果[1] 汤泽生[2] 何兴金[2] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]四川师范学院生物系,四川南充637002

出　　处：《四川大学学报（自然科学版）》2001年第6期899-904,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金 (30 170 5 5 7);四川省应用基础研究基金

摘　　要：突变大麦类囊体膜吸收光谱的蓝区峰值明显高于野生型 ;A683 /A652 比值较高 ;CD谱信号较弱 ,这说明其捕光色素蛋白复合物发生了变化 .SDS PAGE结果表明 ,突变大麦的Rubisco的水平与野生型一致 ,但在类囊体膜上分子量为 2 4～A comparative study of chloroplast ultrastructure, absorption spectrum and SDS-PAGE analysis was made in a chlorophyll-less barley mutant and its wildtype. The results showed that the mutant barley has lower Chl and carotenoid contents but higher Chl a/b ratio.NYB chloroplast possesses less thylakoids and grana stacks.As compared to the wild type barley, the light absorption of NYB thylakoids was much higher within 450～480 nm and 600～640 nm.SDS-PAGE results indicated that there is no differences in Rubisco level but a bigger decrease in LHCⅡ apoproteins between mutant and its wildtype.

关 键 词：大麦 突变体 叶绿体 结构功能 生化特性 叶绿素 光合膜 类囊体膜 

分 类 号：S512.301[农业科学—作物学] Q945.11[生物学—植物学]