人参皂甙对脂多糖诱导小鼠肺泡巨噬细胞分泌肿瘤坏死因子的作用及机制探讨  

Effect and mechanism of action of Ginsinoside on secretion of TNF-α by mouse alveolar macrophages

作　　者：刘轩[1] 魏育林[1] 李波[2] 李亚俊[1] 金恩源[1] 王学静[3] 

机构地区：[1]中日友好临床医学研究所中西医结合室,北京100029 [2]北京大学医学部基础医学系,北京100083 [3]中日友好医院检验科,北京100029

出　　处：《中日友好医院学报》2001年第2期83-86,共4页Journal of China-Japan Friendship Hospital

摘　　要：目的 :研究人参皂甙Rg1和Rb1对脂多糖 (LPS)诱导小鼠肺泡巨噬细胞 (AM )过度分泌肿瘤坏死因子(TNF α)的影响 ,并探讨人参皂甙的作用机制。方法 :体外培养小鼠AM ,分为对照组、LPS组、Rg1+LPS组、Rg1组和Rb1+LPS组、Rb1组。分别收集培养细胞及上清液 ,测定上清TNF α水平、上清LPS水平以及细胞中I κB表达情况。结果 :LPS可使AM分泌大量TNF α(P <0 .0 1) ,并使I κB表达明显减少 (P <0 .0 1)。Rg1+LPS组与LPS组相比 ,上清TNF α水平显著下降 (P <0 .0 1) ,细胞内I κB表达明显增加 (P <0 .0 1)。Rg1+LPS组上清游离LPS水平明显高于LPS组 (P <0 .0 5 )。Rb1的作用结果与Rg1相似。 结论 :人参皂甙Rg1和Rb1在体外能抑制AM过量分泌TNF α ,其可能作用机制为通过阻断LPS与AM膜的结合 ,抑制LPS诱导的细胞内I κB的低表达 ,从而使TNF α的分泌水平下降。Objective:To investigate the effect of Ginsinoside Rg1 and Rb1 on the excess secretion of TNF α by mouse alveolar macrophages(AM)stimulated by lipopolysaccharide(LPS)and study the mechanism of the action.Methods:With the mouse AMs cultured in vitro,the levels of tumor necrosis factor α(TNF α)in cell culture supernatants(ELISA),the expression of I κB in AMs(immunocytochemistry)and the levels of LPS in cell culture supernatants(Quantitative Limus Assay)were observed.Results:The levels of TNF α were significantly increased and the expression of I κB in AMs was significantly decreased in LPS group in comparison with that in control group( P <0.01).The levels of TNF α were much lower and the expression of I κB was much higher in Rg1+LPS group compared with the LPS group ( P <0.01).The levels of LPS in supernatants was much more higher in Rg1+LPS group than that in LPS group ( P <0.05). Rb1+LPS group had the similar results as the Rg1+LPS group did.Conclusion: Ginsinoside Rg1 and Rb1 could suppress the excess secretion of TNF α.The mechanism of suppression was probably that Ginsinoside could block the combination of LPS with AM membrane,and restrain the low expression of I κB and production of TNF α induced by LPS.

关 键 词：人参皂甙 脂多糖 巨噬细胞 肿瘤坏死因子 

分 类 号：R965[医药卫生—药理学]