表皮细胞生长因子通过诱导皮肤干细胞分化加速受创表皮再生的研究  

EPIDERMAL GROWTH FACTOR STIMULATES TISSUE REPAIR IN SKIN THROUGH SKIN STEM CELL ACTIVATION

作　　者：付小兵[1] 孙晓庆[1] 孙同柱[1] 董永洪[1] 顾小曼[1] 陈伟[1] 盛志勇[1] 

机构地区：[1]解放军第304医院,北京100037

出　　处：《中国修复重建外科杂志》2002年第1期31-35,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家重大基础研究规划 ( G19990 5 42 0 4);国家杰出青年科学基金资助项目 ( 395 2 5 0 2 4);全军"九五"重点医学科研基金资助项目 ( 98Z0 90 )

摘　　要：目的　从皮肤干细胞增殖分化角度研究表皮细胞生长因子 (EGF)加速受创皮肤再生的机制。方法　 8例经 EGF治疗创面于治疗后 8天及 14天对创面边缘部进行活检取材。用常规病理与 SP免疫组织化学法研究 β1 整合素、角蛋白 19(K19)、角蛋白 14(K14)以及角蛋白 10 (K10 )在不同修复阶段修复表皮的表达特征。另选7例同期未经 EGF治疗创面为对照。结果　常规病理学检查与对照创面相比 ,EGF治疗创面后 8天可见表皮层增厚 ,表皮脚增粗 ,治疗后 14天以上特征更为明显。SP免疫组织化学对 β1 整合素与 K19染色表明 ,EGF治疗 8天的创面表皮干细胞与短暂扩充细胞不仅数量多 ,而且体积增大 ,治疗后 14天创面再生表皮的棘细胞层中有干细胞岛出现。对照治疗后 8天与 14天创面除有一定数量的 β1 整合素与 K19阳性染色细胞外 ,未见干细胞岛出现。在 EGF治疗和对照治疗创面 ,治疗后 8天及 14天 ,K14、K10表达均见于再生上皮的表层 ,即那些已失去增殖能力与终末分化细胞。结论　Objective To explore the possible mechanisms of skin regeneration through the epidermal stem cells stimulated by epidermal growth factor (EGF). Methods At 8 and 14 days after treatment with EGF, the tissue specimens from 8 skin ulcered patients who were treated with EGF were used to evaluate the distribution and differentiation of epidermal stem cells. The expression of β 1 integrin, keratin 19 (K19), keratin 14(K14) and keratin 10 (K10) in skin was detected with SP immunohistochemical methods. Hematoxylin and eosin staining method were used to observe the tissue structure. Another 7 biopsies from ulcered patients without EGF management were used as the control. Results The results from the hematoxylin and eosin staining showed that the epidermis in EGF treated wounds was thick and the epidermal ridges were enlarged both in 8 and 14 days compared with those in control skin. Immunohistochemical staining from β 1 integrin and K 19 showed that all tissues treated with EGF were rich in epidermal stem cells both in 8 and 14 days. These stem cells were bigger in size and larger in number and localized at the base of the epidermis. In contrast, the positive expression cells of β 1 integrin and K 19 in control group in the same time were scanty. It was found that there were some stem cell islands in epidermis treated with EGF in day 14 and absent from the control group. The expression of K14 and K10 could be observed in those terminally differentiating epidermal cells in both groups. Conclusion The results indicate that the possible mechanisms of skin regeneration stimulated by EGF comes from the mitogenic effects and differiation of skin stem cells. healing

关 键 词：表皮细胞生长因子 皮肤 干细胞 创面愈合 创伤 

分 类 号：R640.2[医药卫生—外科学]