自体皮与异体皮混合移植中的层粘蛋白表达  

THE EXPRESSION OF LAMININ IN THE INTERMINGLED SKIN TRANSPLANTATION OF ALLOGRAFT AND AUTOGRAFT

作　　者：丁韧[1] 贾生贤[1] 赵雁飞[2] 陈玉英[3] 易静[3] 汤雪明[3] 

机构地区：[1]上海第二医科大学瑞金医院烧伤研究所,上海200025 [2]上海第二医科大学病理教研室 [3]上海第二医科大学细胞生物学实验室

出　　处：《中国修复重建外科杂志》2002年第1期36-38,共3页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的　通过对大张异体皮打洞嵌植小块自体皮混合移植皮片中层粘蛋白 (L N )的表达研究 ,探讨自体皮与异体皮混合移植促进 度烫伤创面愈合的机制。方法　清洁级 SD大鼠背部脱毛后 ,造成占体表总面积10 %～ 15 %的 度烫伤。伤后 3天切痂 ,移植大张打洞的异体皮。异体皮移植后第 3天 ,嵌植小块自体皮 ,完成皮肤混合移植。在异体皮移植后 3、5、7、14和 2 1天 ,采用免疫组织化学方法测定移植皮片中 L N的表达。结果　异体皮移植后第 7天出现排异反应 ,表现为异体表皮逐渐被爬行而来的自体表皮所铲除替代 ,异体真皮表面仍可见 L N阳性表达。异体皮移植后第 14天 ,从自体皮岛迁移来的表皮细胞下有零星 L N表达 ,异体皮中的 L N仍基本保持完整。异体皮移植后第 2 1天 ,自体表皮已基本覆盖异体皮区 ,L N表达已基本完整。结论　大张异体皮打洞嵌植小块自体皮混合移植中的异体皮为创面愈合提供了基底膜成分 。Objective To explore the healing mechanism of full thickness wound treating by the intermingled skin transplantation of large sheet allograft with autograft through studying the expression of laminin (LN). Methods Thirty six SD rats with 10% to 15% of total body surface area(TBSA) full thickness were made. After 3 days, the devitalized tissue were excised and transplanted a large sheet of allograft from Wistar rats and islets of autografts were implanted 3 days later. On day 3, 5, 7, 14, 21 after allografting, the expression of LN in the grafts were detected by immunohistochemistry. Results On the 7th day postallografting, LN, which played positive action of epidermal cell adhesion, still retained in the allodermis after the rejection of alloepidermis occurred. On the 14th day postallografting, there appeared scattered LN underneath the epidermal cells migrating from islets of autografts. On the 21th day postallografting, LN in the basement membrane of skin grafts had completely formed. Conclusion The intermingled transplantation of large sheet allograft with autograft may provide components of basement membrane for wound healing, which may help to improve the appearance and function of skin.

关 键 词：自体皮 异体皮 混合移植 层粘蛋白 创面愈合 

分 类 号：R622.1[医药卫生—整形外科]