鸡骨质石化病(骨型白血病)的综合诊断研究  

Studies on the Comprehensive Diagnosis of Avian Osteopetrosis

作　　者：白朝勇[1] 陈白希[1] 

机构地区：[1]华南农业大学兽医诊断研究室,广州510642

出　　处：《畜牧兽医学报》2002年第1期78-84,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目 ( 39770 5 70 )

摘　　要：鸡骨质石化病 (OP)尚缺乏有效可行的现场诊断方法 ,作者用放射学、免疫学、病理组织学、电镜观察和血清生化等方法 ,对 497只鸡 (其中生产场种鸡 2 6 5只、人工诱发OP实验与对照鸡 2 32只 )进行诊断试验比较 ,结果用放射学检出OP阳性率为 13 6 8% (6 8/4 97) ,可疑率为 5 2 3% (2 6 /4 97)。免疫学 (羽髓琼扩 )随机检查 2 14例 ,检出阳性率仅为 4 6 7% (10 /2 14)。病理组织学抽样 2 0例病骨检查 (其中阴性 2例、可疑 6例、不同程度阳性 12例 )诊断结果均与放射学诊断结果一致 ,并证实可疑鸡已有轻度病理改变 ,应视为阳性。抽样 5例 (含 1例阴性 )病骨作电镜观察 ,4例阳性均可见病毒粒子。实验组与对照组 10 2只鸡血清生化学检查 :磷、碱性磷酸酶、球蛋白及总蛋白虽有统计学差异 ,但不足以作为诊断依据。综合各种诊断方法 ,认为放射学的检出率最高 ,具有在生产现场诊断OP的实际价值。Using radiography,immuno agar diffusion,pathologic histodiagnosis,electron microscopy and serum biochemical parameter determination comparison was carried out on 265 chickens in the field and 232 artificially infected with avian myeloblastosis virus in an attempt to overcome the lack of feasible diagnostic method to detect osteopetrosis on the farm.The results showed that radiography was of practical value,the percentage of radiological positive cases was being 13 68% (68/497) and the suspicion rate 5 23%(26/497).But the immuno agar diffusion positive rate was 4 67%(10/214),all the 214 cases being selected at random.The 20 cases subjected to pathologic histodiagnosis,showed 2 negatives,6 suspicious and 12 positive of varying degrees,the result being consistent with that of radiological detection,and verified that the radiologic suspicious cases had mild pathologic changes.There were viral particles seen in 4 radiologic positive cases,and none found in negative cases under the electron microscope.Although there was significant difference in the levels of serum phosphorus,alkaline phosphatase,globulin and total protein between experimental and control groups from 102 chickens,they were not considered enough for diagnosis.

关 键 词：骨型白血病 骨质石化病 放射学 综合诊断 鸡 

分 类 号：S858.31[农业科学—临床兽医学]