硫酸铵盐析在蕲蛇毒凝血酶样酶分离中的应用  

Application of ammonium sulfate salting-out to isolate thrombin-like enzymes from Agkistrodon acutus venom

作　　者：郭春腾[1] 宁千年 薛伟明[1] 傅蓉[1] 饶平凡[1] 

机构地区：[1]福州大学生物工程研究所,福建福州350002 [2]三明汇天生物药业有限公司,福建三明365000

出　　处：《福州大学学报（自然科学版）》2002年第1期122-125,共4页Journal of Fuzhou University(Natural Science Edition)

摘　　要：探讨了硫酸铵沉降对蕲蛇毒中凝血酶样酶的初步分离效果的影响 ,确定出最佳处理条件 :2 .0 %的蕲蛇毒在pH 5 .0及 4 5 %饱和度的硫酸铵溶液中盐析沉降蕲蛇毒中非凝血酶样酶杂蛋白 。The method of ammonium sulfate salting-out to preliminarily isolate thrombin-like enzymes from Agkistrodon acutus venom was applied and the separation effect was discussed in this paper. The optimum treatment to salting-out non-thrombin-like-enzyme components in 2.0% crude venom was determined as 45% ammonium sulfate at pH?5.0. The supernatant is preliminary isolated thrombin-like enzymes. The specific activity of the isolated thrombin-like enzymes is three times as high as rude Agkistrodon acutus venom, and percent recovery of protein is 54.6%.

关 键 词：蕲蛇毒 凝血酶样酶 硫酸铵盐析 色谱分离 沉淀量 凝历活力 PH值 

分 类 号：R996.3[医药卫生—毒理学] Q556.9[医药卫生—药学]