苯胺甲基键合固定相的合成和色谱性能  

Preparation and Evaluation of N-Methylaniline Bonded Silica for High Performance Liquid Chromatography

作　　者：周蓉[1] 范毅[1] 冯钰錡[1] 达世禄[1] 

机构地区：[1]武汉大学化学与分子科学学院,武汉430072

出　　处：《应用化学》2002年第1期66-70,共5页Chinese Journal of Applied Chemistry

摘　　要：研究了苯胺甲基键合硅胶固定相的合成及其色谱性能 .采用元素分析、红外光谱对固定相的组成进行了表征 .以甲醇 /水为流动相考察了该固定相的色谱性能 .该固定相具有较好的疏水作用和电荷转移能力 .多环芳烃在该固定相的保留与正十八烷固定相 ( ODS)柱相似 ;硝基苯类、硝基苯酚类和氯苯类的溶质在该固定相保留则存在着电荷转移、偶极 -偶极作用 .同时对封尾前后该固定相的色谱行为进行了比较 .A new bonded stationary phase, N-methylaniline bonded silica for reversed-phase HPLC was synthesized by reacting N-methylanilinetriethoxylsiliane with silica directly. The packing material was characterized by diffuse reflectance infrared Fourier transform spectrometry and elemental analysis. The chromatographic behavior of hydrophobicity, charge-transfer ability, dipole-dipole and hydrogen bonding interaction have been studied on the bonded stationary phase using methanol-water as binary mobile phase. Compared with octadecyl stationary phase(ODS), the new bonded phase was superior in selectivity and separation rate for PAH and its derivatives, and exhibited the best separation selectivity for acidic aromatic derivatives.

关 键 词：多环芳烃 色谱法 苯胺甲基键合固定相 合成 色谱性能 保留机理 

分 类 号：O657.7[理学—分析化学]