内蒙古砷中毒和台湾乌脚病病区井水中腐植酸性质的比较  

Comparison of humic acid from endemic arsenicosis areas in Inner Mongolia with the Blackfoot disease areas in Taiwan

作　　者：余孝颖[1] 吕锋洲[2] 郑宝山[1] 刘聪桂[3] 黄天祥[4] 夏雅娟 洪清霖 

机构地区：[1]中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳550002 [2]台湾大学医学院生化所 [3]台湾大学地质系 [4]台湾大学医学院内科 [5]内蒙古地方病防治研究所,内蒙古呼和浩特020020 [6]台北医学院

出　　处：《中国地方病学杂志》2002年第1期37-40,共4页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金委员会 ( 494 732 0 2 );中华发展基金委员会 (台北 )资助项目

摘　　要：目的　为了探讨地下水性质与台湾乌脚病区相似的内蒙古砷中毒病区今后是否会出现乌脚病。方法　用 AAS(原子吸收光谱 )、ICP-MS(等离子体发射光谱 -质谱 )、红外、荧光和 TL S(Total lum inescence spec-troscopy)光谱分析了两地水样的理化特征、元素组成、荧光强度和各种谱学特征 ;还对两病区水样中提取的腐植酸样品进行了 Ames试验和脂质过氧化反应的试验。结果　发现内蒙砷中毒病区地下水属于 HCO3 -Na和 HCO3 -Cl-Na型 ,平均 p H值为 7.91,为弱碱性还原环境。两地水样中的砷浓度和腐植酸的荧光强度均较高 ,荧光强度与砷浓度、p H值和总溶解固体 (TDS)呈正相关关系。且两地水中腐植酸的荧光光谱性质也相似。但是它们的红外光谱和 TL S光谱有差异。这些差异在生物学效应上表现为 :内蒙腐植酸具有较强的引发脂质过氧化反应的能力 ,而台湾腐植酸对 TA98(± S9)菌株具有致突变作用。结论　内蒙砷病区Objective In order to shed light on the pathogenesis of the Blackfoot disease (BD) with the concern as to whether the disease may eventually occur in arsenicosis affected areas in Inner Mongolia.Methods The physical and chemical properties of humic acids (HA) extracted from drinking waters from the endemic arsenicosis areas in Inner Mongolia and from BD areas in Taiwan are studied by using AAS (atomic absorption spectrophotometry), ICP MS (inductively coupled plasma emission mass spectrometry),IR(Infrared spectroscopy),FR (fluorescence spectrometry ) and TLS (total luminescence spectroscopy). Ames test and lipid peroxidation experiments were also conducted on these HA samples.Results It is found that water samples from the two regions are considerably high in As with strong fluorescence and apparent positive correlations between As content, fluorescence, pH and total dissolved solids (TDS). The water samples are similar in fluorescence spectra but differ somewhat in IR and TLS between the two regions. The difference may be a reflection of the difference in radicals and structure of the HA owing to different hydrogeological conditions, and may also be related to the difference in their biological effects, i. e., HA from Inner Mongolia have a stronger ability to cause lipid peroxidation while HA from Taiwan exhibits a more prominent effect of mutation with respect to TA98(±S9).Conclusions It is considered that BD is unlikely to be expected in Inner Mongolia at least in the near future.

关 键 词：砷中毒 腐植酸 光谱分析 致突变性 脂质过氧化反应 乌脚病 内蒙古 台湾 井水 

分 类 号：R599.9[医药卫生—内科学] R123.1[医药卫生—临床医学]