柯萨奇B3病毒中国分离株VP1基因序列分析  

Sequence analysis of the VP1 Gene of Coxsackievirus B3 Chinese Strain

作　　者：钟照华[1] 田野[2] 郭淑元[1] 赵文然[3] 赵铁强[2] 安毅[2] 孟繁超[2] 谷鸿喜[1] 鲁凤民 叶鸿瑁 

机构地区：[1]哈尔滨医科大学微生物教研室,黑龙江哈尔滨150086 [2]哈尔滨医科大学第一临床医学院心内科,黑龙江哈尔滨150001 [3]哈尔滨医科大学克山病研究所,黑龙江哈尔滨150086 [4]北京大学第三临床医学院临床病毒研究室,北京100083

出　　处：《中国地方病学杂志》2002年第1期8-10,共3页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金 ( 39570 74 4);黑龙江省自然科学基金( D- 96 0 2)

摘　　要：目的　分析柯萨奇 B3病毒 (CVB3 )中国分离株编码主要中和抗原的 VP1基因序列。方法　用逆转录 PCR技术扩增 CVB3中国分离株的 VP1基因 ,通过 TA克隆法构建 p CR2 .1-VP1并进行核苷酸序列的测定分析。结果　测序发现 CVB3中国分离株与 CVB3 Nancy株 VP1基因均编码 2 93个氨基酸 ,二者存在 5 9个核苷酸差异 ,其中 10处影响到氨基酸的编码 ,其余均为同义变异。结论　 CVB3中国分离株与 Nancy株 VP1基因序列存在一定的差异 。Objective To identify the sequence of VP1 gene which encodes the main neutralizing antigen of Coxsackievirus B3 Chinese strain.Methods The VP1 gene of CVB3 was amplified by reverse transcript polymerase chain reaction (RT PCR) and pCR2.1 VP1 was constructed by TA cloning strategy.The recombinant plasmid had been analyzed by DNA sequencing.Results Although the VP1 gene of CVB3 Chinese strain encoded 293 amino acids that was similar to the VP1 gene of CVB3 Nancy strain, there were 59 differences between them. Most of the variations were nonsense mutations. Only 10 amino acids changed due to the variations.Conclusions There were differences between VP1 gene of Chinese strain and Nancy strain, our results may be helpful to the research of immunological characteristics of CVB3 Chinese strain.

关 键 词：柯萨奇病毒B 序列测定 VP1基因 

分 类 号：R373.23[医药卫生—病原生物学] Q523.8[医药卫生—基础医学]