抗CEA单克隆抗体碘标记方法研究  

Studies of the radioiodination methods for anti-CEA monoclonal antibody

作　　者：陆丽仪[1] 韩章淑 戴黛红 林汉[2] 田毅 

机构地区：[1]北京师范大学 [2]中国医学科学院放射医学研究所

出　　处：《核技术》1989年第11期648-652,共5页Nuclear Techniques

摘　　要：本文报道用几种碘标记方法(Iodosen、ChT、NBS、Bolton-Hunter试剂等)制备了^(125)I(^(131)I)-抗CEA单克隆抗体。产品通过Sephacex G-50(或-75)柱凝胶过滤纯化后,经纸层析和高压液相层析法鉴定,其放化纯度达到使用要求;用免疫放射双抗体夹心法测定,标记抗体能保持良好的免疫活性。同时我们对上述几种碘标方法进行比较研究,结果NBS法标记率最高,达98％,Iodogen法免疫活性保持最好,与CEA的净结合数达9960cpm(3ng CEA/250μl)。The preparation of 125I(131I)-anti-CEA monoclonal antibody by using several radio-iodination methods (Iodogen, ChT, NBS, and Bolton-Hunter reagent etc.) was described. Purification of the product was carried out by the Sephadex G-50 (or 75) gel-filtration column. Its radiochemical purity checked by paper chromatography and HPLC was satisfactory for use. The labelled antibody showed good immunoreactivity, when it was measured by IRMA-bi-antibody sandwich method. The comparative studies for above several radioiodination methods were carried out, among which the iodination efficiency of NBS method was the best (98%) while the immunoreactivity of lodogen method was the best and specific binding of labelled antibody to CEA was 9960 cpm (3ng CEA/ 250 μl).

关 键 词：单克隆抗体 碘标记法 IODOGEN法 

分 类 号：Q5－3[生物学—生物化学]