偶然分支杆菌对各种物理因素的抵抗力及在不同环境的存活情况  

Resistance of Mycobacterium fortuitum against varied physical factors and its survival in different surroundings

作　　者：庄宝玲[1] 吴春敏[1] 林光宇[1] 

机构地区：[1]福建省南平市卫生防疫站,353000

出　　处：《中华结核和呼吸杂志》2001年第11期679-681,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：福建省南平市科委资助项目

摘　　要：目的　观察偶然分支杆菌对各种物理因素的抵抗力以及在不同环境中的存活情况。方法　将偶然分支杆菌的菌悬液分别加到各种灭菌的载体上 (试管、平皿、纸片 ) ,置不同的物理环境 (高温、高压紫外线、干燥、日照 )中经不同时间处理 ,将处理后的标本进行培养观察细菌存活情况 ,以了解其对各种物理因素的抵抗力。同时取一定量菌悬液分别加到各种环境介质中 (自来水、湿沙土、干燥沙土 ) ,置室温于不同时间连续取样培养观察细菌生长情况 ,以了解在不同环境介质中存活的情况。结果　偶然分支杆菌需 6 0℃ 135min灭活 ,80℃ 6 0min灭活 ,10 0℃ 5min灭活 ;高压灭菌 (6 8kg12 1℃、4 5kg 115℃ ) 5min均能灭菌 ;日照下暴晒 1h就能灭活 ;但紫外线的强度为 10 4 5 μW cm需5h才能灭活。偶然分支杆菌在自来水中存活 2 7个月以上 ;在干燥的环境中、湿沙土中和干沙土中存活1 5年以上 ,随着时间的延长只是其菌量呈下降趋势。结论　偶然分支杆菌对高温的抵抗力较低 ,而对紫外线的抵抗力很强。在不同环境介质中有较强的耐受性 。Objective To observe the resistance of Mycobacterium fortuitum against some physical factors and its survival in different surroundings Methods Bacterium solution of Mycobacterium fortuitum was added to the carriers, then it was treated in different interval of time and physical surroundings (high temperature, maximum pressure in ultraviolet ray, dry, sunshine) The treated specimen was cultured to observe the resistance of Mycobacterium fortuitum against physical factors At the same time, some of bacterium solution were mixed into materials in different surroundings (water, wet soil, and dry soil) The specimen was cultured constantly in different time under the room temperature to observe the growth of Mycobacterium fortuitum in different surroundings Results Mycobacterium fortuitum was killed at 60℃ in 135 minutes, 80℃ in 60 minutes,100℃ in 5 minutes, and high pressure in 5 minutes (6 8 kg at 121℃, 4 5 kg at 115℃) It can also be killed under hot sunshine in one hour and under the ultraviolet ray at 105 5 μW/cm Mycobacterium fortuitum can exist in water over 27 months, in wet and dry soil and in dry surroundings for more than one and a half year With the extending of time, quantities of Mycobacterium fortuitum showed a trend of decline Conclusions Mycobacterium fortuitum possesses low resistance against high temperature, but higher resistance against ultraviolet ray The germ shows stronger endurance in different surroundings and can exist for a long time

关 键 词：偶然分支杆菌 环境暴露 抵抗力 存活 

分 类 号：R52[医药卫生—内科学]