肾小球上皮细胞分泌肾上腺髓质素抑制系膜细胞增殖  

Adrenomedullin Produced by Glomerular Epithelial Cell Inhibits Mesangical Cell Proliferation

作　　者：刘学光[1] 陈广平[2] 张志刚[1] 郭慕依[1] 

机构地区：[1]复旦大学基础医学院病理学教研室,上海200032 [2]美国北海岸LIJ健康机构放射肿瘤学部,纽约11030

出　　处：《复旦学报（医学版）》2001年第5期375-377,共3页Fudan University Journal of Medical Sciences

基　　金：上海市教委重点学科项目基金 (B990 80 2 );复旦大学校CMB基金资助

摘　　要：目的　探讨肾上腺髓质素 (AM)及其受体CRLR在培养的大鼠肾小球上皮细胞 (GEC)和系膜细胞(MsC)的表达 ,以及AM对MsC生长的影响。方法　采用RT PCR法分别制备AM及CRLR探针 ,Northernblot法检测其在GEC和MsC的表达 ,[3H]胸腺嘧啶掺入法测定MsC增殖情况。结果　AM仅表达于GEC ,不表达于MsC ,而其受体CRLR的表达则相反。含有AM的GEC培养上清可抑制MsC生长 ,而CRLR阻断剂CGRP8-37可部分消除此抑制作用。结论　GEC可通过合成和分泌AM抑制MsC增殖 ,提示AM作为一种重要的旁分泌因子 ,以保持肾小球微环境稳定 。Purpose: To investigate the expression of adrenomedullin (AM) and its receptor calcitonin-receptor-like receptor (CRLR) on renal cells and the possible effect of AM on mesangial cell proliferation. Methods: RT-PCR was used to prepare the AM and CRLR probes, Northern blot used to detect the expression of AM and CRLR, [3H] thymidine incorporation used to measure cell proliferation. Results: AM was produced by glomerular epithelial cell (GEC), but not by mesangial cell (MsC), however, AM receptor CRLR was only expressed on MsC. GEC conditioned medium containing AM inhibited MsC growth and AM receptor blockade CGRP8-37 may partially decrease this inhibitory effect. Conclusions: It is suggested that AM as an important glomerular paracrine regulator is involved in glomerular integrity and inflamatory process.

关 键 词：肾小球上皮细胞 肾小球系膜细胞 肾上腺髓质素 聚合酶链反应 Northern BLOT法 肾小球肾炎 炎症 细胞增殖 

分 类 号：R692.31[医药卫生—泌尿科学]