芦丁和黄芩甙在清除α-羟乙基过氧自由基过程中生成的过氧化氢的测定方法(1)  

MEASUREMENT OF THE G VALUES OF HYDROGEN PEROXIDE IN THE REACTIONS OF RUTIN AND BAICALIN WITH α-HYDROXYL ETHYL PEROXYL RADICALS

作　　者：张福根[1] 尹毅宁[1] 吴季兰[1] 

机构地区：[1]北京大学技术物理系,北京100871

出　　处：《辐射研究与辐射工艺学报》2001年第4期263-269,共7页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金 (2 970 2 0 0 3)资助

摘　　要：用动态吸附的方法对γ辐照芦丁和黄芩甙的空气饱和乙醇体系中产生的过氧化氢进行分离 ,测定了辐照生成的过氧化氢的G值 ,得到了与前人不同的结果 ,并分析了产生分歧的原因。提出并确立了正确的方法 :准确测定黄酮类化合物辐照体系中的过氧化氢值 ,必须先用柱色谱法将过氧化氢从辐照体系中分离出来再进行测定 ,再扣除黄酮类化合物因自氧化而产生的过氧化氢的量。采用 6 .93Gymin- 1的剂量率 ,测得 1× 10 - 3 mol·dm- 3 的芦丁和黄芩甙乙醇溶液中 ,经γ辐照后产生的过氧化氢G值分别为 5 .5± 0 .2和 7.3± 0 .2。与纯乙醇相比 ,辐照后过氧化氢的G值增加。Irradiated rutin and baicalin ethanol solutions saturated with air were eluted through alumina columns and the G values of hydrogen peroxide generated in the solutions were measured. Different results from former works were obtained and the reasons of the difference were discussed. A precise method was established as follows: hydrogen peroxide should be separated from flavonoids by passing the flavonoids ethanol solutions through alumina columns before the measurement and the amount of hydrogen peroxide generated from self-oxidation of the flavonoids should be deducted. Under a dose rate of 6.93Gy min -1 , the G values of hydrogen peroxide in γ irradiated rutin and baicalin ethanol solutions saturated with air were determined to be 5.5±0.2 and 7.3±0.2, separately. After irradiation of rutin- and baicalin-O 2-ethanol solutions, the G values of hydrogen peroxide were increased comparing with that of pure ethanol-O 2 solution.

关 键 词：芦丁 黄芩甙 过氧化氢 测定方法 α-羟乙基过氧自由基 黄酮类化合物 

分 类 号：O626.31[理学—有机化学]