新型连续棒状疏水色谱柱的制备及其色谱性能研究  

STUDY ON THE PREPARATION OF NEW CONTINUOUS ROD COLUMN AND ITS CHROMATOGRAPHIC BEHAVIOUR

作　　者：卫引茂[1] 黄晓冬[1] 陈强[2] 耿信笃[1] 

机构地区：[1]西北大学现代分离科学研究所现代分离科学陕西省重点实验室,西安710069 [2]宝鸡文理学院化学系,陕西宝鸡721000

出　　处：《离子交换与吸附》2001年第5期310-315,共6页Ion Exchange and Adsorption

基　　金：国家自然科学基金 (No. 39880003);陕西省教委专项科学基金

摘　　要：采用连续棒柱制备技术,制备了一种甲基丙烯酸缩水甘油酯-甲基丙烯酸丁酯-已二酸二甲基丙烯酸酯三元共聚物连续棒疏水色谱柱。考察了聚合条件对聚合物孔结构及介质疏水性的影响。应用该色谱柱对标准蛋白混合样及基因工程药物g-INF和胰岛素的前驱体Pro-insulin提取液进行了分离纯化,效果良好。The continuous rod hydrophobic interaction chromatographic column consisting of poly (glycidyl methacrylate-co-butyl methacrylate-co-ethylene dimethacrylate) was prepared by using the polymeric synthesis technique of continuous rod. The effects of polymerization conditions on the polymer抯 pore structure and the hrdrophobility of the rod were investigated. Good results were achieved when the rod column was used in the separation and purification of five standard protein mixture and recombinant human proinsulin and human interferon-g produced from E. colin in biotechnology.

关 键 词：疏水色谱 共聚物基质 连续棒色状色谱柱 蛋白质 制备 色谱性能 分离 纯化 

分 类 号：O657.7[理学—分析化学] O658[理学—化学]