DNA光复活作用机理的研究进展  

Recent Progress in Studies of Mechanism for DNA Photoreactivation

作　　者：宋钦华[1] 郭庆祥[1] 

机构地区：[1]中国科学技术大学化学系,合肥230026

出　　处：《化学进展》2001年第6期428-435,共8页Progress in Chemistry

基　　金：国家自然科学基金 (批准号 :30 0 0 0 0 36 );中国科学技术大学青年科学基金资助项目

摘　　要：环丁烷型嘧啶二聚体 ( Pyr<>Pyr)是太阳光中紫外线造成 DNA损伤的主要光化学产物。DNA光复活酶 (或称光解酶 )能够利用可见光裂解二聚体的环丁烷环而修复 DNA。本文对 DNA光复活过程中的光解酶对 Pyr<>Pyr的识别和光催化 Pyr<>Pyr裂解反应进行了综述 ,介绍了 DNA光解酶的结构、DNA的主要 UV光化学产物。较详尽地评述了国际上在光解酶催化二聚体裂解的途径以及模型研究方面的最新进展 。Cyclobutane pyrimidine dimers (Pyr<>Pyr) are the major DNA photoproducts induced by the UV radiation. Photoreactivating enzyme (DNA photolyase) repairs DNA by utilizing the energy of visible light to break the cyclobutane ring of the dimer. In this paper, binding and splitting mechanism of pyrimidine dimer by DNA photolyase were reviewed. The structure of DNA photolyases and the UV induced DNA photoproducts were introduced. In particular. recent research progress in photorepair mechanism of the photolyase was discussed.

关 键 词：光复活作用 DNA 光解酶 环丁烷型嘧啶二聚体 分子识别 光修复作用 紫外线 辐射损伤 

分 类 号：Q523[生物学—生物化学] Q557