蜂王浆超氧化物歧化酶分离纯化及部分性质研究  

PURIFICATION AND CHARACTERIZATION OF SUPEROXIDE DISMUTASE FROM ROYAL JELLY

作　　者：闵丽娥[1] 刘克武[1] 杨守忠[1] 刘晓雯[1] 黄裕林[2] 喻东 

机构地区：[1]四川大学生命科学学院,四川成都610064 [2]四川大学化学学院,四川成都610064

出　　处：《化学研究与应用》2001年第5期567-569,共3页Chemical Research and Application

摘　　要：本文采用硫酸铵盐析、SephedexG 15 0柱层析从蜂王浆中分离出了超氧化物歧化酶 (SOD)。经聚丙烯酰胺凝胶电泳显示单一蛋白区带。此酶比活力为 1887.4U/mg ,纯化倍数为 5 2 4.3,回收率为 12 .47% ,最适pH 8.3 ,最适温度为 2 5℃。Mn2 + ,Cu2 + ,Ba2 + ,Mg2 + 对此酶有激活作用 ,Zn2 + 则具有抑制作用。过氧化氢和乙醇 氯仿实验表明 :蜂王浆中的SOD属Cu/ZnCopper Zinc superoxide dismutase was purified from royal jelly by precipitation with ammonium sulfate and Sephadex G150 in 12.47% yield by a purification factor of 524.3. The specific activity of SOD was estimated as 1887.4U/mg protein. The purified enzyme showed a single band on PAGE. The results from treatment with H 2O 2 or ethanol/chloroform,indicated that the enzyme was Cu/Zn SOD. Its optimum pH was 8.3 and optimum temperature,25℃.Mn 2+ ,Cu 2+ ,Ba 2+ and Mg 2+ activate the enzyme and Zn 2+ inhibits the enzyme.

关 键 词：蜂王浆 SOD 分离 纯化 超氧化物歧化酶 保健作用 

分 类 号：S896.3[农业科学—特种经济动物饲养] Q554.9[农业科学—畜牧兽医]