简易荧光仪共振光散射光谱法测定蛋白质的研究  

STUDY OF PROTEIN WITH SIMPLE FLUORESCENCE-SPECTROPHOTOMETER BY RESONANCE LIGHT SCATTERING

作　　者：王林[1] 王照丽[2] 李树伟[2] 

机构地区：[1]重庆渝西学院化学系,重庆永川402168 [2]四川师范大学化学与生命科学学院,四川成都610066

出　　处：《化学研究与应用》2001年第5期591-593,共3页Chemical Research and Application

摘　　要：The article studied the resonance light scattering of protein red alizarin with the simple fluorescence spectrophotometer first.In Britton Robinson butter of 4.10,the red alizarin combined with proteins by intermolecular forces to form complexes,causing an enhance of resonance light scattering(RLS) at maximum wavelength of about 360nm.Based on the enhancement of resonance light scattering a novel method for the determination of proteins using red alizarin was developed.Though it affected by the limit of the filter of spectrophotometer the experiment used the wavelength of incident light doesn’t lie in the maximum wavelength,the method has better linear range within 0～10.0μg/ml,determination limits being 0.04μg/ml.The method had been used to determine total proteins in human serum samples and with accuracy and torelant to many foreign substance.The article studied the resonance light scattering of protein red alizarin with the simple fluorescence spectrophotometer first.In Britton Robinson butter of 4.10,the red alizarin combined with proteins by intermolecular forces to form complexes,causing an enhance of resonance light scattering(RLS) at maximum wavelength of about 360nm.Based on the enhancement of resonance light scattering a novel method for the determination of proteins using red alizarin was developed.Though it affected by the limit of the filter of spectrophotometer the experiment used the wavelength of incident light doesn't lie in the maximum wavelength,the method has better linear range within 0～10.0μg/ml,determination limits being 0.04μg/ml.The method had been used to determine total proteins in human serum samples and with accuracy and torelant to many foreign substance.

关 键 词：蛋白质 茜素红 共振光散射光谱 测定 荧光分光光度计 分析 

分 类 号：Q51[生物学—生物化学] O629.73[理学—有机化学]