双黄连粉针剂中黄芩苷的高效毛细管电泳-电导法测定  

Quantitative Determination of Baicalin in Shuanghuanglian Powder-injectable by High Performance Capillary Electrophoresis -Conductivity Detection

作　　者：郑一宁[1] 谢天尧[1] 莫金垣[1] 韦寿莲[2] 邓光辉[2] 

机构地区：[1]中山大学化学与化工学院,广东广州510275 [2]广西民族学院化学化工系,广西南宁530006

出　　处：《分析测试学报》2001年第6期21-24,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目 (29675033) ;广东省自然科学基金资助项目 (001237)

摘　　要：建立了双黄连粉针剂中黄芩苷含量测定的高效毛细管电泳电导法 ;以未涂层融硅毛细管 (75μm×50cm)为分离柱 ,Tris-H3BO3 为电泳介质 (15mmol/LTris ,1.5mmol/LH3BO3) ,在18kV、pH8.5的碱性环境下 ,柱端电导检测双黄连粉针剂中的黄芩苷含量 ;重点探讨了缓冲溶液的种类、浓度、分离电压、进样时间对检测的影响 ;该法的线性范围为10～720mg/L,检出限为2.5mg/L;结果表明该法简便、快速、结果准确 ,适用于含黄芩苷的制剂含量的测定。A method for the determination of baicalin in Shuanghuanglian powder _ injectable was established by high performance capillary electrophoresis with conductivity detection. Based on the mode of capillary zone electrophoresis, baicalin was successfully separated from other components and determined within 5 minutes under following selected conditions: uncoated fused silica capillary(75 μm×50 cm), Tris-H3BO3 buffer solution(pH=8.5, 15 mmol/L Tris, 1.5 mmol/L H3BO3), 18 kV operation voltage. The effect of kinds of buffer solutions, buffer solution concentration, operation voltage, sampling time were discussed in detail. A linear range of 10～720 mg/L and a detection limit of 2.5 mg/L for baicalin were obtained. The method proved to be convenient, rapid and well repeatable, and can be used for the quality control of preparations with baicalin.

关 键 词：高效毛细管电泳 电导检测器 双黄连粉针剂 黄芩 中药 黄芩苷 药物含量测定 

分 类 号：R284.1[医药卫生—中药学]