应用PCR技术检测小型兽类肾组织中钩端螺旋体DNA的研究  

Leptospire DNA Detection from Kidneys of the Small Animals by PCR

作　　者：王俊[1] 顾丽丽[1] 胡万富[1] 刘红[1] 王建军[1] 

机构地区：[1]安徽省卫生防疫站,230061

出　　处：《安徽预防医学杂志》2001年第6期405-406,共2页Anhui Journal of Preventive Medicine

摘　　要：目的 :应用PCR技术对安徽省 7个不同地区所获的小型兽类肾组织中携带的钩端螺旋体DNA进行检测。结果 :安徽省的小型兽类携带钩体DNA检出率为 9.41% (16 / 170 ) ,不同地区间检出率有所不同 ,个别地区啮齿类动物携带钩体DNA检出率高达 19.0 5 % (12 / 6 3) ,而有的地区则未检出 ;不同的小兽之间携带钩体DNA的检出率也有所不同 ,黑线姬鼠为 12 .96 % (14/ 10 8) ,褐家鼠为 5 .88% (2 / 34 ) ,其余 4种啮齿类动物则未检出。结论 :应用PCR技术能准确及时地从宿主动物的肾脏组织中检测出钩体DNA的存在 ,其高度特异性、敏感性是鼠肾培养及鼠血MAT的检测不可比拟的 ,用其来分析钩体病的流行因素、流行特征和地理分布以及为今后扩大钩体病调查 。This paper reported leptospire DNA detection from kidneys of the small animals captured from 7 districts in AnHui province. The results indicated that total detection rate of DNA was 9.14%(16/170), 12.96% (14/108) from Aa and 5.88%(2/34) from Rn respectively, ranging from 19.05% (12/63) to 0 in different areas. It was accurate and promptly to detect DNA from the kidneys of the host animals by PCR, which was peculiar and sensitive compared with the other methods, i.e. culture kidney of the rodent, MAT. It offered new methods with analyzing epidemic factors and features and geographical distribution and expending the investigation for Leptospirosis.

关 键 词：钩端螺旋体 DNA 聚合酶链反应 

分 类 号：R514.4[医药卫生—内科学]