输卵管妊娠患者生殖道巨细胞病毒和沙眼衣原体的测定  

Detection of genital tract cytomegalovirus and chlamydia trachomatis infection in patients with tubal pregnancy

作　　者：金杭美[1] 林俊[1] 徐开红[1] 吕时铭[1] 陈晓端[1] 蔡挺[1] 

机构地区：[1]浙江大学医学院附属妇产科医院妇科,杭州310006

出　　处：《中华妇产科杂志》2001年第10期600-602,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　探讨巨细胞病毒和沙眼衣原体感染与输卵管妊娠的关系。方法　应用酶联免疫吸附试验法检测 95例输卵管妊娠患者 (异位妊娠组 )和 42例行附件切除术、无输卵管妊娠史的卵巢囊肿患者 (对照组 )血清中抗巨细胞病毒IgM ,应用聚合酶链反应技术测定生殖道组织中巨细胞病毒gH基因和沙眼衣原体热休克蛋白基因。结果　异位妊娠组抗巨细胞病毒IgM阳性 14例 ,阳性率为15 % ,对照组阳性 1例 ;巨细胞病毒gH基因阳性 18例 ,阳性率为 19% ,对照组则 2例阳性 ,两组比较差异有显著性 (P <0 0 5 )。异位妊娠组沙眼衣原体热休克蛋白基因阳性 2 5例 ,阳性率为 2 6 % ,对照组则阳性 2例 ,两组比较 ,差异有显著性 (P <0 0 5 )。Objective To investigate the influence of genital tract cytomegalovirus and chlamydia infection on the incidence of tubal pregnancy. Methods Ninety five women with tubal pregnancy (study group) and 42 women with ovarian cysts (control group) were selected for this study. Serum cytomegalovirus (CMV) IgM was identified by enzyme link immunosorbent assay (ELISA). Cervical secretions, endometrium, and salpinx tissue were tested for CMV gH gene and chlamydia heat shock protein (HSP) gene by nest polymerase chain reaction (PCR) and PCR respectively. Results CMV IgM was positive in 14 (15%) women with tubal pregnancy, and 1 (2%) in the control group. CMV gH gene was detected in 18 women (19%) and chlamydia HSP gene in 25 (26%) of the tubal pregnancy group, and 2 (5%) and 2 (5%) in the control group respectively. There were significant differences between the study and the control group ( P <0 05) with respect to detection of CMV IgM, CMV gH gene and chlamydia HSP gene. Conclusions CMV and chlamydia trachomatis genital tract infection were more common in women with tubal pregnancy than that in the control group. Genital CMV and chlamydia trachomatis infection may associate with an increased incidence of tubal pregnancy.

关 键 词：妊娠 输卵管 巨细胞病毒属 衣原体 沙眼 热休克蛋白质类 

分 类 号：R714.22[医药卫生—妇产科学]