小麦幼穗培养的植株再生及变异  

PLANT REGENERATION FROM GROWING TIPS CULTURES OF WHEATS AND ITS VARIATION

作　　者：郑企成[1] 朱耀兰[1] 陈文华[1] 

机构地区：[1]中国农业科学院原子能利用研究所

出　　处：《核农学报》1989年第3期129-136,共8页Journal of Nuclear Agricultural Sciences

摘　　要：对11个基因型冬小麦护颖分化期的幼穗进行诱导培养,愈伤组织诱导率接近100％。经120余天继代培养后再生植株,再生率达73.7—100％。将SC1代植株移入田间,在219株植株中观察到育性、株高、抽穗期、穗形、芒形、籽粒(大小、形状和颜色)、颖色和腊性等广泛变异。经测定,籽粒α-淀粉酶和醇溶蛋白也出现变异。11个不同基因型的变异频率不等,各性状的变异频率也不相同。Growing tips of 11 genotypic wheats at the glume differentiation stage were cultured on MS medium. The callus induction frequency was about 100%. Plant regeneration frequency was 73.7-100% after subcultured for 120 days.Regenerated plant were planted in the field.Extensive variation was observed among 219 regenerants. Variation characters included fertility, height, heading date, spike shape, awns,grain characters, glume colour, waxiness, a-amylase and gliadin proteins.Frequencies and compositions of variation among the 11 genotypes were different.

关 键 词：小麦 再生植株 变异 

分 类 号：S512.101[农业科学—作物学]