单细胞凝胶电泳对男性精液质量的评价  

Evaluation on Semen Quality by Single Cell Gel Electrophoresis

作　　者：丁晓萍[1] 闫素文[1] 张宁[1] 鲁海欧[1] 张黎[1] 汤洁[1] 王喜良[1] 

机构地区：[1]解放军202医院全军优生优育研究中心,沈阳110003

出　　处：《中国公共卫生》2001年第11期970-971,共2页Chinese Journal of Public Health

摘　　要：目的　采用单细胞凝胶电泳的方法评价短波辐射对男性精液质量的影响 ,早期发现精子的亚临床损伤 ,及时提出干预措施 ,保证人口素质真正意义上的提高。方法　采用常规精液分析和单细胞凝胶电泳相结合的方法分别检测短波接触者的精液质量 ,以判断短波辐射对男性生殖功能的影响。结果　短波辐射暴露组精液分析无变化 ,但单细胞凝胶电泳 -DNA链断裂分析显示Ⅰ级彗星率明显高于对照组。结论　提示长期接受短波辐射人员的精液分析包括精子数量、精子活力和精子活率等影响不大 ,但可见精子DNA链断裂增多 。Objective To evaluate the effects of short wave radiation on male semen quality by single cell gel electrophoresis(SCGE).The method makes it possible to find sub clinical injury on sperm and to propose protection measures in time to insure promoting population quality realistically.Methods To detect the semen quality from men exposed to short-wave radiation by routine semen analysis and sperm single cell gel electrophoresis(SCGE).Results No changes on semen analysis can be observed in short-wave radiation group,but the SCGE analysis showed first class comet cell percentage increased significantly than control group.Conclusion Analyses on persons exposed to short-wave radiation with long time showed slight changes on sperm concentration motility,and the rate of viability but sperm DNA strand breakage was obvious.The micro-structure(ie,sub-clinical)change can be detected by SCGE.

关 键 词：短波辐射 精液质量 DNA链断裂 单细胞凝胶电泳 检测 生殖健康 

分 类 号：R122.4[医药卫生—环境卫生学] R697[医药卫生—公共卫生与预防医学]