NO与肝脏缺血再灌注损伤预处理保护效应  

NO AND MECHANISMS OF PRECONDITIONING AGAINST ISCHEMIA /REPERFUSION INJURY IN RAT LIVER

作　　者：王占明[1] 高德明[1] 鲁建国[1] 马庆九[1] 吴金生[1] 

机构地区：[1]第四军医大学唐都医院普通外科,西安710038

出　　处：《中国现代医学杂志》2001年第12期14-16,共3页China Journal of Modern Medicine

摘　　要：目的 :研究缺血预处理对SD大鼠肝脏缺血再灌注损伤保护机制中NO的作用 ;方法 :建立大鼠肝脏缺血再灌注模型并进行缺血预处理 ,实验分组如下 :假手术组 (Sham -operation ,S组 ) ;缺血再灌注组 (IschemicReperfusion ,I/R组 ) ;缺血预处理组 (IschemicPreconditioning ,PC组 ) ;预处理加左旋硝基精氨酸组 (Precondi tioning +L -NNA ,PC +L组 ) ,各组再灌注后检测血清丙氨酸转氨酶 (ALT)及丙二醛 (MDA)含量 ,肝组织石蜡切片HE染色及电镜观察 ;结果 :PC组ALT及MDA含量明显低于I/R组 (P <0 .0 1) ,肝组织损伤程度明显减轻 ;PC +L组在 0 - 3h阶段ALT和MDA含量与I/R组无显著区别 (P >0 .0 5 ) ,而在 6～ 48h阶段则显著低于I/R组 (P <0 .0 1)但仍高于PC组 (P <0 .0 5 )。结论 :缺血预处理对大鼠肝脏缺血再灌注损伤具有明显保护作用 ,0～ 3h为早期保护效应 。Objective:To study late preconditioning(PC) against ischemia/reperfusion(I/R) injury in rat liver.Methods:We divided 168 SD rats into four groups:①S group:n=42,sham-operated control;②I/R group:n=42, subjected to reperfusion after 70-minute ischemia;③PC group:n=42,two cycles of 5-minute ischemia followed by 5-minute reperfusion were performed before I/R;④PC+L group:n=42,L-NNA was administered before preconditioning,then 70 min ischemia was performed .Followed by continuous reperfusion,serum ALT,MDA,NO,NOS were examined in all groups during 0～48h.Results:Serum ALT and MDA in both I/R and PC+L group markedly increased than in PC group( P <0.01) at any point after reperfusion.There was no significant difference between I/R group and P+L group during 0～3h( P >0.05),but during 6～48h( P <0.01),ALT and MDA increased in PC+L vs PC group ( P <0.05) and markedly lower than I/R group ( P <0.01).Conclusions:NO might be participate in the protection against liver ischemia/reperfusion injury and NO might protect liver in early preconditioning.

关 键 词：缺血预处理 延迟保护效应 缺血再灌注损伤 肝脏 

分 类 号：R657.3[医药卫生—外科学]