大鼠Fn剪接异型体分布及皮肤损伤后表达研究  

In vivo distribution and expression after skin injury of fibronectin splicing variants

作　　者：陈忆九[1] 刘宁国[1] 黄晓华[1] 李莉[1] 

机构地区：[1]司法部司法鉴定科学技术研究所,上海200063

出　　处：《法医学杂志》2001年第4期193-195,共3页Journal of Forensic Medicine

基　　金：上海市科委基金资助项目(No.002512079)

摘　　要：目的研究成年大鼠、新生幼鼠纤维连接蛋白剪接异型体EⅢA、EⅢB体内分布及皮肤切创、挫伤后表达情况,以期为法医学损伤经历时间研究探索新指标及选择阳性对照。方法组织匀浆后抽提总RNA,逆转录聚合酶链反应(RT-PCR)技术扩增目标条带。结果(1)正常皮肤EⅢA+、EⅢB+不表达,伤后18hEⅢA+位点高丰度表达,EⅢB+位点变化不明显,两种致伤方式表达结果差别不大;(2)成年大鼠各器官分布情况依次为脑、心、肝。结论EⅢA+可作为皮肤损伤研究的敏感指标。Objective In order to develop new criteria and to select positive controls for forensic medicine, experiments on in vivo distribution and expression after skin wound of fibronectin splicing variants, EⅢA and EⅢB were studied. Methods Rat tissues were homogenized to extract total RNA which was then amplified by RT-PCR technique. Results (1)EⅢA+ and EⅢB+ of normal skin were not expressed, whereas EⅢA+ after 18 hours' injury was expressed abundantly. No obvious charge of EⅢB+ was detected. The differences between results of using two methods of injury were not significant.(2)The distribution of fibronectin variants in different adult organs were in the following order: brain >heart >liver. Conclusion EⅢA+ may be used as a sensitive criterion for research of skin injury.

关 键 词：皮肤损伤 纤维连接蛋白EⅢA 可变剪接 法医鉴定 

分 类 号：D919.4[医药卫生—法医学] R89[政治法律—法学]