PDGF-β,PDGFR-β,TGF-β_1,bFGF在创伤愈合过程中的表达变化与损伤时间关系的研究  

A preliminary study on the changes of expression of PDGF-β, PDGFR-β, TGF-β1, TGFR, bFGF and its relationship with the wound age in wound healing

作　　者：王慧君[1] 阮海根[2] 黄光照[3] 

机构地区：[1]第一军医大学病理学教研室法医学组,广东广州510515 [2]武汉市公安局刑科所,湖北430015 [3]华中科技大学同济医学院法医学系,湖北武汉430030

出　　处：《法医学杂志》2001年第4期198-201,共4页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(No.39870764);(广东省科委攻关项目(2KM05404S;2000)

摘　　要：目的研究细胞因子在创伤及修复过程中的表达变化与损伤时间(伤口年龄)的关系。方法用免疫组织化学技术和图像分析技术,对实验大鼠不同损伤时间的生前伤(0.5～168ham)及死后伤(0.5～6hpm)皮肤创缘组织中细胞因子和受体的表达变化进行了研究。结果在生前伤,PDGF-β,PDGFR-β,TGF-β1,bFGF等细胞因子在上皮细胞的表达于损伤后0.5ham即开始增强,其中以24～96ham反应最强。TGF-β1,bFGF以及PDGF-β除了在上皮细胞的表达外,还大量出现在肉芽组织的巨噬细胞和成纤维细胞内,亦以24～96ham最明显,而死后损伤组上述因子仅在0.5～3h以内有弱表达,3h后则均无任何表达。结论PDGF-β,PDGFR-β,TGF-β1,bFGF在创伤愈合过程中的变化及特点与损伤时间的关系可作为推断损伤时间的免疫病理学标志。Objective To explore the relationship between the expression change of cytokines and the wound age during the healing process of rats skin wound .Methods Immunohistochemical and image-analysis methods were performed on vital skin wounds(after incision 0.5-168h am) and postmortem damage(after incision 0.5-6h pm).Results The expression of the cytokines PDGF-β,PDGFR-β, TGF-β1, and bFGF in the epithelial cells was already enhanced since 0.5h am after damage and their strongest expression reaction was seen at 24-96h am. In addition, the expression of PDGF-β, PDGFR-β, TGF-β1 and bFGF was also found in the macrophages and the fibroblasts of the granulation tissue, and the expression changes in the postmortem damage group showed that the skin tissue within 0.5-3h after incision showed immunohistochemical changes but weakly expression and 3h thereafter no any change was found. Conclusion The expression characteristics of the above mentioned cytokines in wound repair should be related to the wound age and it reminds therefore that they may be used as immunohistochemical criteria for accurate determining the wound age.

关 键 词：创伤修复 细胞因子的表达 损伤时间的推断 法医鉴定 

分 类 号：D919.4[医药卫生—法医学] R89[政治法律—法学]